Department of Cardiothoracic Surgery, Beijing Luhe Hospital of Capital Medical University, Beijing, China.
J Clin Lab Anal. 2019 May;33(4):e22847. doi: 10.1002/jcla.22847. Epub 2019 Feb 4.
Vitamin D is an indispensable molecule for human health. Wide ranges of diseases are linked with vitamin D deficiencies. Role of vitamin D in chronic heart failure has been demonstrated in different populations; however, reports are limited in Chinese population. Vitamin D exerts its effect through vitamin D receptor and variants in vitamin D receptor (VDR) gene are shown to affect vitamin D signaling. In the present study, we hypothesized that both vitamin D levels and VDR variants could be associated with the development of chronic heart failure.
We enrolled 145, chronic heart failure patients those admitted to Department of Cardiothoracic Surgery, Beijing Luhe Hospital of Capital Medical University and fulfilled NYHA inclusions criteria. In addition, ninety healthy subjects from similar geographical location were enrolled as healthy controls. Plasma levels of vitamin D were quantified by ELISA. VDR variants (BsmI, ApaI, TaqI, and FokI) were genotyped by PCR-RFLP.
Plasma levels of vitamin D were significantly lower in chronic heart patients compared to healthy controls. Heterozygous and minor allele for FokI and TaqI polymorphisms were significantly higher in heart failure patients when compared to healthy controls. In addition, combined analysis of vitamin D levels and VDR mutants revealed association of vitamin D deficiencies and VDR mutants with chronic heart failure.
The results of the present investigation showed an important role of vitamin D and VDR variants with chronic heart failure.
维生素 D 是人体健康不可或缺的分子。广泛的疾病与维生素 D 缺乏有关。维生素 D 在不同人群中的慢性心力衰竭中的作用已经得到证明；然而，在中国人群中的报道有限。维生素 D 通过维生素 D 受体发挥作用，维生素 D 受体（VDR）基因中的变异被证明会影响维生素 D 信号。在本研究中，我们假设维生素 D 水平和 VDR 变异都与慢性心力衰竭的发展有关。
我们招募了 145 名慢性心力衰竭患者，这些患者入住首都医科大学附属北京潞河医院心胸外科，并符合 NYHA 纳入标准。此外，还招募了 90 名来自相似地理位置的健康受试者作为健康对照组。通过 ELISA 定量测定血浆维生素 D 水平。通过 PCR-RFLP 对 VDR 变异（BsmI、ApaI、TaqI 和 FokI）进行基因分型。
与健康对照组相比，慢性心力衰竭患者的血浆维生素 D 水平显著降低。与健康对照组相比，FokI 和 TaqI 多态性的杂合子和次要等位基因在心力衰竭患者中明显更高。此外，维生素 D 水平和 VDR 突变体的联合分析显示，维生素 D 缺乏症和 VDR 突变体与慢性心力衰竭有关。
本研究结果表明维生素 D 和 VDR 变异与慢性心力衰竭有重要关系。
