一种简单而稳健的流式细胞术检测方法在成熟外周 T 细胞淋巴瘤中的快速克隆性检测中的应用。
Utility of a Simple and Robust Flow Cytometry Assay for Rapid Clonality Testing in Mature Peripheral T-Cell Lymphomas.
机构信息
Department of Pathology, Brigham and Women's Hospital, Boston, MA.
Harvard Medical School, Boston, MA.
出版信息
Am J Clin Pathol. 2019 Apr 2;151(5):494-503. doi: 10.1093/ajcp/aqy173.
OBJECTIVES
Flow cytometry immunophenotyping is limited by poor resolution of T-cell clones. A newly described antibody was recently used to distinguish normal peripheral blood T cells from malignant T-cell clones. Here, we evaluate this antibody as a new diagnostic tool for detecting T-cell clonality in mature peripheral T-cell lymphomas.
METHODS
Immunostaining for the T-cell receptor β chain constant region 1 (TRBC1) along with routine T-cell markers was performed on 51 peripheral blood and two bone marrow samples submitted to the flow cytometry laboratory for suspected T-cell malignancy.
RESULTS
TRBC immunophenotyping identified malignant T-cell clones with 97% sensitivity and 91% specificity. Findings correlated with molecular T-cell clonality testing. In cases with equivocal molecular results, TRBC1 immunophenotyping provided additional diagnostic information.
CONCLUSIONS
TRBC1 flow cytometric immunophenotyping is a robust and inexpensive method for identifying T-cell clonality that could easily be incorporated into routine flow cytometric practice.
目的
流式细胞术免疫表型分析受到 T 细胞克隆分辨率差的限制。最近描述了一种新的抗体，用于区分正常外周血 T 细胞和恶性 T 细胞克隆。在这里，我们评估该抗体作为一种新的诊断工具，用于检测成熟外周 T 细胞淋巴瘤中的 T 细胞克隆性。
方法
对 51 例外周血和 2 例骨髓样本进行 T 细胞受体β链恒定区 1（TRBC1）免疫染色，这些样本被送到流式细胞实验室进行疑似 T 细胞恶性肿瘤的检测。
结果
TRBC 免疫表型分析具有 97%的敏感性和 91%的特异性，可识别恶性 T 细胞克隆。结果与分子 T 细胞克隆性检测相关。在分子结果不确定的情况下，TRBC1 免疫表型分析提供了额外的诊断信息。
结论
TRBC1 流式细胞术免疫表型分析是一种强大且经济的方法，可用于识别 T 细胞克隆性，可轻松纳入常规流式细胞术实践。
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