MTM Research Centre, School of Science and Technology, Örebro University, Örebro, Sweden.
Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Environ Sci Pollut Res Int. 2019 Mar;26(9):9079-9088. doi: 10.1007/s11356-019-04281-4. Epub 2019 Feb 4.
Plastic is able to sorb environmental pollutants from ambient water and might act as a vector for these pollutants to marine organisms. The potential toxicological effects of plastic-sorbed pollutants in marine organisms have not been thoroughly assessed. In this study, organic extracts from four types of plastic deployed for 9 or 12 months in San Diego Bay, California, were examined for their potential to activate the aryl hydrocarbon receptor (AhR) pathway by use of the H4IIE-luc assay. Polycyclic aromatic hydrocarbons (PAH), including the 16 priority PAHs, were quantified. The AhR-mediated potency in the deployed plastic samples, calculated as bio-TEQ values, ranged from 2.7 pg/g in polyethylene terephthalate (PET) to 277 pg/g in low-density polyethylene (LDPE). Concentrations of the sum of 24 PAHs in the deployed samples ranged from 4.6 to 1068 ng/g. By use of relative potency factors (REP), a potency balance between the biological effect (bio-TEQs) and the targeted PAHs (chem-TEQs) was calculated to 24-170%. The study reports, for the first time, in vitro AhR-mediated potencies for different deployed plastics, of which LDPE elicited the greatest concentration of bio-TEQs followed by polypropylene (PP), PET, and polyvinylchloride (PVC).
塑料能够从周围水中吸附环境污染物，并可能成为这些污染物向海洋生物转移的载体。塑料吸附污染物对海洋生物的潜在毒理学影响尚未得到彻底评估。在这项研究中，使用 H4IIE-luc 测定法，研究了在加利福尼亚州圣地亚哥湾部署 9 或 12 个月的四种类型的塑料中的有机提取物，以评估其激活芳香烃受体 (AhR) 途径的潜力。定量了多环芳烃（PAH），包括 16 种优先 PAH。以生物-TEQ 值计算，部署的塑料样本中的 AhR 介导效力范围从聚对苯二甲酸乙二醇酯（PET）中的 2.7 pg/g 到低密度聚乙烯（LDPE）中的 277 pg/g。部署样本中 24 种 PAH 的总和浓度范围为 4.6 至 1068 ng/g。通过相对效力因子（REP），计算了生物效应（生物-TEQs）和目标 PAH（化学-TEQs）之间的效力平衡，比例为 24-170%。该研究首次报告了不同部署塑料的体外 AhR 介导效力，其中 LDPE 产生的生物-TEQs 浓度最高，其次是聚丙烯（PP）、PET 和聚氯乙烯（PVC）。
