Choi Jun Ho, Park Sang Kyun, Park Mi Kyung, Kang Shin Ae, Lee Dain, Song So Myong, Kim Hye Jin, Park Sung Hee, Kim Eun-Min, Yu Hak Sun
Department of Parasitology, School of Medicine, Pusan National University, Immunoregulatory Therapeutics group in Brain Busan 21 project, Republic of Korea.
Department of Environmental Medical Biology and Arthropods of Medical Importance Resource Research Bank, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
Parasitol Int. 2019 Jun;70:41-45. doi: 10.1016/j.parint.2019.01.010. Epub 2019 Feb 1.
Recent studies have attempted to treat autoimmune diseases using Trichuris suis. whipworm) eggs. Large quantities of eggs can be obtained efficiently by collecting from the feces of the porcine hosts rather than by extracting from the female worm uterus. However, it is difficult to process large amounts of feces using the current methods. In the present study, we propose a method to collect the eggs from bulk feces more efficiently. Collecting the eggs using washing meshes (25 μm sieve) yields 65.7% (56.0-70.7) of eggs (median, min-max) from 100 g feces. Our method, which uses ethyl acetate and simulated gastric fluid, yielded 91.4% (91.4-94.0) of the eggs from 100 g feces into the separated aqueous solution. Egg collection using simulated gastric fluid (SGF) method was also 60 min faster than that using the sieve method. As the SGF used in the experiment is a strongly acidic reagent with a pH of 1-2, embryonation of the eggs was induced by the rapid pH change. As a result, 37.1% (8.0-77.8) of the eggs had embryonated two months after SGF stimulation. Using the developed method, we could process the feces quickly and efficiently. Furthermore, after purification, egg embryonation could be induced without any harmful reagent treatment. This method is expected to be helpful for further research using Trichuris suis eggs.
最近的研究尝试使用猪鞭虫的虫卵来治疗自身免疫性疾病。通过从猪宿主的粪便中收集，而非从雌虫子宫中提取，可以高效地获得大量虫卵。然而，使用当前方法处理大量粪便存在困难。在本研究中，我们提出了一种更高效地从大量粪便中收集虫卵的方法。使用清洗筛网（25微米筛）从100克粪便中收集虫卵，虫卵回收率为65.7%（中位数，最小值-最大值：56.0-70.7）。我们使用乙酸乙酯和模拟胃液的方法，从100克粪便中获得了91.4%（91.4-94.0）的虫卵进入分离出的水溶液中。使用模拟胃液（SGF）法收集虫卵比使用筛网法快60分钟。由于实验中使用的SGF是一种pH值为1-2的强酸性试剂，pH值的快速变化诱导了虫卵的胚胎发育。结果，在SGF刺激两个月后，37.1%（8.0-77.8）的虫卵发生了胚胎发育。使用所开发的方法，我们可以快速且高效地处理粪便。此外，经过纯化后，无需任何有害试剂处理即可诱导虫卵胚胎发育。该方法有望对使用猪鞭虫卵的进一步研究有所帮助。
