Bondarenko Natalya, Glotova Anna, Nassonova Elena, Masharsky Alexey, Polev Dmitry, Smirnov Alexey
Department of Invertebrate Zoology, Faculty of Biology, St. Petersburg State University, Universitetskaya nab. 7/9, 199034 St. Petersburg, Russia.
Department of Invertebrate Zoology, Faculty of Biology, St. Petersburg State University, Universitetskaya nab. 7/9, 199034 St. Petersburg, Russia.
Eur J Protistol. 2019 Apr;68:80-87. doi: 10.1016/j.ejop.2019.01.005. Epub 2019 Jan 15.
We present a complete sequence and describe the organization of the mitochondrial genome of the amoeba Paravannella minima (Amoebooza, Discosea, Vannellida). This tiny species represents a branch at the base of Vannellida tree, to the moment being its earliest-branching lineage. The circular mitochondrial DNA of this species has 53,464 bp in length and contains 30 protein-coding genes, 2 ribosomal RNAs, 23 transfer RNAs, and 15 open reading frames. This genome is significantly longer and contains more protein-coding genes than any yet sequenced mitochondrial genome of vannellid amoebae. Unlike the previously sequenced mitochondrial genomes of Vannellida, which should be translated using the "Table 4" (the mold, protozoan, and coelenterate mitochondrial code), that of P. minima can be properly translated using the universal genetic code.
我们展示了变形虫微小副瓦氏变形虫(变形虫纲、盘变形虫目、瓦氏变形虫科)线粒体基因组的完整序列,并描述了其组织结构。这个微小的物种代表了瓦氏变形虫科谱系树基部的一个分支,目前是其分支最早的谱系。该物种的环状线粒体DNA长度为53,464 bp,包含30个蛋白质编码基因、2个核糖体RNA、23个转运RNA和15个开放阅读框。这个基因组比任何已测序的瓦氏变形虫线粒体基因组都长得多,并且包含更多的蛋白质编码基因。与之前测序的瓦氏变形虫线粒体基因组不同,后者应使用“表4”(霉菌、原生动物和腔肠动物线粒体密码子表)进行翻译,而微小副瓦氏变形虫的线粒体基因组可以使用通用遗传密码进行正确翻译。