Bondarenko Natalya I, Nassonova Elena S, Mijanovic Olja, Glotova Anna A, Kamyshatskaya Oksana G, Kudryavtsev Alexander A, Masharsky Alexey E, Polev Dmitrii E, Smirnov Alexey V
Department of Invertebrate Zoology, Faculty of Biology, St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
Laboratory of Cytology of Unicellular Organisms, Institute of Cytology RAS, Tikhoretsky ave. 4, St. Petersburg, 194064, Russia.
J Eukaryot Microbiol. 2018 Nov;65(6):820-827. doi: 10.1111/jeu.12523. Epub 2018 May 7.
Mitochondrial genome sequence of Vannella croatica (Amoebozoa, Discosea, Vannellida) was obtained using pulse-field gel electrophoretic isolation of the circular mitochondrial DNA, followed by the next-generation sequencing. The mitochondrial DNA of this species has the length of 28,933 bp and contains 12 protein-coding genes, two ribosomal RNAs, and 16 transfer RNAs. Vannella croatica mitochondrial genome is relatively short compared to other known amoebozoan mitochondrial genomes but is rather gene-rich and contains significant number of open reading frames.
通过脉冲场凝胶电泳分离环状线粒体DNA,然后进行下一代测序,获得了克罗地亚范内拉变形虫(变形虫门、盘状亚门、范内拉目)的线粒体基因组序列。该物种的线粒体DNA长度为28,933碱基对,包含12个蛋白质编码基因、两个核糖体RNA和16个转运RNA。与其他已知的变形虫线粒体基因组相比,克罗地亚范内拉变形虫的线粒体基因组相对较短,但基因丰富,包含大量开放阅读框。