通过 RNA 测序对乳腺癌前哨淋巴结中 microRNA 表达的鉴定，以提高诊断准确性。

Identification of microRNA expression in sentinel lymph nodes from patients with breast cancer via RNA sequencing for diagnostic accuracy.

机构信息

Department of Ultrasonography, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.

Department of Breast Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.

出版信息

J Gene Med. 2019 Apr;21(4):e3075. doi: 10.1002/jgm.3075. Epub 2019 Mar 3.

DOI:10.1002/jgm.3075

PMID:30716792

Abstract

BACKGROUND

Sentinel lymph node (SLN) property assessment (with or without metastasis) is important when deciding the surgery for breast cancer; however, the current diagnosis of SLN metastasis remains to be studied. microRNAs (miRNAs) have been confirmed previously as a molecular marker for the diagnosis, development and prognosis of tumors. However, the detailed role of miRNAs in the diagnosis of SLN metastasis has not been reported.

METHODS

The present study aimed to explore the potential use of miRNAs in the diagnosis of SLN using RNA sequencing (RNA-seq) and a quantitative real-time polymerase chain reaction (qRT-PCR) to compare the expression profiles of miRNAs in patients with breast cancer with or without SLN metastasis.

RESULTS

The RNA-seq results revealed that 1993 miRNAs were differentially expressed in patients with breast cancer with SLN metastasis. Among these miRNAs, 1960 were up-regulated and 33 were down-regulated. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses revealed that these differentially expressed miRNAs were associated with tumor growth and metastasis and were also predicted to regulate a series of tumorigenesis and metastasis genes. In particular, the most differentially expressed miRNAs were validated by qRT-PCR, such that miR-200a-3p and miR-96-5p were up-regulated and miR-1-3p and miR-486-3p were down-regulated in patients with breast cancer with SLN metastasis.

CONCLUSIONS

The findings of the present study suggest that there is an association of miRNAs with SLN metastasis and also that miRNAs function as biomarkers with respect to the choice of therapy and disease prognosis.

摘要

背景

前哨淋巴结 (SLN) 性质评估（有无转移）对于决定乳腺癌手术方式非常重要；然而，目前 SLN 转移的诊断仍有待研究。microRNAs（miRNAs）已被证实是肿瘤诊断、发展和预后的分子标志物。然而，miRNAs 在 SLN 转移诊断中的详细作用尚未报道。

方法

本研究旨在通过 RNA 测序（RNA-seq）和定量实时聚合酶链反应（qRT-PCR），探索 miRNA 用于诊断 SLN 的潜在用途，比较有或无 SLN 转移的乳腺癌患者的 miRNA 表达谱。

结果

RNA-seq 结果显示，有 SLN 转移的乳腺癌患者中存在 1993 个差异表达的 miRNA。其中 1960 个上调，33 个下调。GO 和 Kyoto Encyclopedia of Genes and Genomes 通路富集分析表明，这些差异表达的 miRNA 与肿瘤生长和转移有关，也被预测调控一系列肿瘤发生和转移基因。特别是，通过 qRT-PCR 验证了最具差异表达的 miRNA，miR-200a-3p 和 miR-96-5p 上调，miR-1-3p 和 miR-486-3p 下调。