Food Science and Technology Department, Faculty of Agriculture, Alexandria University, 21545, El-Shatby, Alexandria, Egypt; Department of Materials Science & Engineering, University of Toronto, 5 King's College Road, Toronto, Ontario M5S 3G8, Canada.
Department of Materials Science & Engineering, University of Toronto, 5 King's College Road, Toronto, Ontario M5S 3G8, Canada.
Food Res Int. 2019 Feb;116:660-667. doi: 10.1016/j.foodres.2018.08.093. Epub 2018 Aug 29.
A novel highly selective lutein-producing marine microalga was isolated and identified using high throughput screening (HTS). Phylogenetic analysis based on 18S rRNA gene sequence revealed it belongs to a microalgae class (Trebouxiophyceae), and is closely related to Auxenochlorella spp. The novel strain was designated as Auxenochlorella sp. strain LEU27. Based on HPLC-DAD and HPLC-MS analyses, strain LEU27 produced a total of 1203.9 ± 98 μg g dry cells of carotenoids, including a remarkable amount (996.6 ± 98 μg g dry cells) of pure lutein under heterotrophic growth conditions. This is the first report that describes a natural, highly selective lutein-producing marine microalga isolated by HTS approach.
高通量筛选（HTS）分离和鉴定了一株新型高选择性产叶黄素海洋微藻。基于 18S rRNA 基因序列的系统发育分析表明，它属于微藻门（Trebouxiophyceae），与Auxenochlorella spp.密切相关。新型菌株被命名为Auxenochlorella sp. strain LEU27。基于 HPLC-DAD 和 HPLC-MS 分析，菌株 LEU27 在异养生长条件下共产生了 1203.9±98μg·g-1 干细胞的类胡萝卜素，其中叶黄素的含量非常高（996.6±98μg·g-1 干细胞）。这是首次通过 HTS 方法分离得到天然、高选择性产叶黄素海洋微藻的报道。
