Zhang Qiu-Hua, Wang Zheng, Wang Yao-Qiang, Zhao Yi-Lin, Su Hai-Jia
Beijing Key Laboratory of Bioprocess, and Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029 People's Republic of China.
3 Biotech. 2024 Oct;14(10):244. doi: 10.1007/s13205-024-04097-1. Epub 2024 Sep 25.
In this study, a novel colorimetric screening method for identifying menaquinone-7 (MK-7) producing strains was established using potassium permanganate. To our knowledge, this method represents the first direct screening methodology for the identification of MK-7 producing strains. Utilizing this screening method, a new MK-7 producing strain, GSA-184, was identified from the soil of the Tibetan Plateau. Under the optimized fermentation medium (50 g/L glycerol, 30 g/L yeast extract powder, 100 g/L soybean peptone, 1 g/L KHPO, and 1 g/L MnSO), the production of MK-7 was increased to 25.7 mg/L. Additionally, the maximum production of MK-7 reached 36.46 mg/L after 48 h in a 5-L fermenter.
The online version contains supplementary material available at 10.1007/s13205-024-04097-1.
在本研究中，建立了一种使用高锰酸钾鉴定产甲基萘醌-7（MK-7）菌株的新型比色筛选方法。据我们所知，该方法是鉴定产MK-7菌株的首个直接筛选方法。利用此筛选方法，从青藏高原土壤中鉴定出一株新的产MK-7菌株GSA-184。在优化的发酵培养基（50 g/L甘油、30 g/L酵母提取物粉、100 g/L大豆蛋白胨、1 g/L KHPO和1 g/L MnSO）中，MK-7的产量提高到了25.7 mg/L。此外，在5-L发酵罐中培养48 h后，MK-7的最大产量达到36.46 mg/L。
在线版本包含可在10.1007/s13205-024-04097-1获取的补充材料。
