Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China.
College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 100039, China.
Mar Drugs. 2019 Feb 1;17(2):84. doi: 10.3390/md17020084.
A novel, cold-adapted, and acid-base stable manganese superoxide dismutase (Ps-Mn-SOD) was cloned from hadal sea cucumber sp. The dimeric recombinant enzyme exhibited approximately 60 kDa in molecular weight, expressed activity from 0 °C to 70 °C with an optimal temperature of 0 °C, and resisted wide pH values from 2.2⁻13.0 with optimal activity (> 70%) at pH 5.0⁻12.0. The m and max of Ps-Mn-SOD were 0.0329 ± 0.0040 mM and 9112 ± 248 U/mg, respectively. At tested conditions, Ps-Mn-SOD was relatively stable in divalent metal ion and other chemicals, such as β-mercaptoethanol, dithiothreitol, Tween 20, Triton X-100, and Chaps. Furthermore, the enzyme showed striking stability in 5 M urea or 4 M guanidine hydrochloride, resisted digestion by proteases, and tolerated a high hydrostatic pressure of 100 MPa. The resistance of Ps-Mn-SOD against low temperature, extreme acidity and alkalinity, chemicals, proteases, and high pressure make it a potential candidate in biopharmaceutical and nutraceutical fields.
从深海海参中克隆出一种新型的耐冷、酸碱稳定的锰超氧化物歧化酶（Ps-Mn-SOD）。二聚体重组酶的分子量约为 60 kDa，在 0°C 到 70°C 之间表现出活性，最适温度为 0°C，能抵抗宽 pH 值范围为 2.2⁻13.0，在 pH 5.0⁻12.0 时活性最佳（>70%）。Ps-Mn-SOD 的 m 和 max 分别为 0.0329 ± 0.0040 mM 和 9112 ± 248 U/mg。在测试条件下，Ps-Mn-SOD 在二价金属离子和其他化学物质（如β-巯基乙醇、二硫苏糖醇、Tween 20、Triton X-100 和 Chaps）中相对稳定。此外，该酶在 5 M 尿素或 4 M 盐酸胍中表现出显著的稳定性，能抵抗蛋白酶的消化，并能耐受 100 MPa 的高压。Ps-Mn-SOD 对低温、极端酸碱、化学品、蛋白酶和高压的耐受性使其成为生物制药和营养保健品领域的潜在候选者。
