Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China; University of Chinese Academy of Sciences, Beijing 100039, China.
Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China.
Int J Biol Macromol. 2019 Nov 1;140:998-1005. doi: 10.1016/j.ijbiomac.2019.08.089. Epub 2019 Aug 10.
Superoxide dismutases (SODs) are one of the most important antioxidant enzymes against oxidative damage. In the present study, we cloned and expressed a novel and stable Cu, Zn-SOD from a hadal sea cucumber Psychropotes longicauda (i.e., Pl-Cu, Zn-SOD). The purified recombinant enzyme was intracellular, dimeric with the Mr. of approximately 38 kDa, with the expressed activity from 0 °C to 60 °C at an optimal temperature of 20 °C and 30 °C and maximum activity at the pH of 8.0. The Km and Vmax values of Pl-Cu, Zn-SOD were 0.041 ± 0.004 mM and 1450.275 ± 36.621 U/mg, respectively. At tested conditions, Pl-Cu, Zn-SOD was relatively stable in chemicals, such as β-ME, EDTA, Tween 20, Triton X-100, and Chaps, especially in urea and guanidine hydrochloride, which can resist protease hydrolysis and tolerate high hydrostatic pressure of 100 MPa and 2 M NaCl. All these properties make Pl-Cu, Zn-SOD a candidate in the biopharmaceutical and nutraceutical fields, and help us better understand the adaptation mechanism of hadal area.
超氧化物歧化酶（SODs）是对抗氧化损伤的最重要的抗氧化酶之一。在本研究中，我们从深海海参 Psychropotes longicauda 中克隆并表达了一种新型且稳定的 Cu，Zn-SOD（即 Pl-Cu，Zn-SOD）。纯化的重组酶为细胞内二聚体，Mr 约为 38 kDa，表达活性在 0°C 至 60°C 之间，最佳温度为 20°C 和 30°C，在 pH 值为 8.0 时达到最大活性。Pl-Cu，Zn-SOD 的 Km 和 Vmax 值分别为 0.041±0.004 mM 和 1450.275±36.621 U/mg。在测试条件下，Pl-Cu，Zn-SOD 在化学品中相对稳定，如 β-ME、EDTA、Tween 20、Triton X-100 和 Chaps，尤其是在尿素和盐酸胍中，能够抵抗蛋白酶水解，并耐受 100 MPa 和 2 M NaCl 的高静水压力。所有这些特性使 Pl-Cu，Zn-SOD 成为生物制药和营养保健品领域的候选物，并帮助我们更好地理解深海区域的适应机制。
