Schnabel Uta, Handorf Oliver, Yarova Kateryna, Zessin Björn, Zechlin Susann, Sydow Diana, Zellmer Elke, Stachowiak Jörg, Andrasch Mathias, Below Harald, Ehlbeck Jörg
Plasma Bioengineering, Leibniz Institute for Plasma Science and Technology e.V., Felix-Hausdorff-Straße 2, 17491 Greifswald, Germany.
School of Food Science and Environmental Health, College of Sciences and Health, Technological University Dublin, Cathal Brugha Street, D01 HV58 Dublin, Ireland.
Foods. 2019 Feb 2;8(2):55. doi: 10.3390/foods8020055.
The synergistic antimicrobial effects of plasma-processed air (PPA) and plasma-treated water (PTW), which are indirectly generated by a microwave-induced non-atmospheric pressure plasma, were investigated with the aid of proliferation assays. For this purpose, microorganisms (, , , sporulated ) were cultivated as monocultures on specimens with polymeric surface structures. Both the distinct and synergistic antimicrobial potential of PPA and PTW were governed by the plasma-on time (5⁻50 s) and the treatment time of the specimens with PPA/PTW (1⁻5 min). In single PTW treatment of the bacteria, an elevation of the reduction factor with increasing treatment time could be observed (e.g., reduction factor of 2.4 to 3.0 for ). In comparison, the combination of PTW and subsequent PPA treatment leads to synergistic effects that are clearly not induced by longer treatment times. These findings have been valid for all bacteria ( > = ). Controversially, the effect is reversed for endospores of . With pure PPA treatment, a strong inactivation at 50 s plasma-on time is detectable, whereas single PTW treatment shows no effect even with increasing treatment parameters. The use of synergistic effects of PTW for cleaning and PPA for drying shows a clear alternative for currently used sanitation methods in production plants. Highlights: Non-thermal atmospheric pressure microwave plasma source used indirect in two different modes-gaseous and liquid; Measurement of short and long-living nitrite and nitrate in corrosive gas PPA (plasma-processed air) and complex liquid PTW (plasma-treated water); Application of PTW and PPA in single and combined use for biological decontamination of different microorganisms.
借助增殖试验研究了由微波诱导的非大气压等离子体间接产生的等离子体处理空气（PPA）和等离子体处理水（PTW）的协同抗菌作用。为此，将微生物（、、、芽孢杆菌）作为单一培养物在具有聚合物表面结构的标本上培养。PPA和PTW独特的以及协同的抗菌潜力均受等离子体开启时间（5⁻50秒）和标本用PPA/PTW处理的时间（1⁻5分钟）的控制。在对细菌进行单次PTW处理时，可以观察到随着处理时间的增加还原系数升高（例如，对而言还原系数为2.4至3.0）。相比之下，PTW与随后的PPA处理相结合会产生协同效应，而较长的处理时间显然不会诱导这种效应。这些发现对所有细菌（> = ）均有效。有争议的是，对于的芽孢杆菌，效果则相反。采用纯PPA处理时，在等离子体开启时间为50秒时可检测到强烈的失活，而单次PTW处理即使增加处理参数也无效果。利用PTW的清洁协同效应和PPA的干燥协同效应为生产工厂目前使用的卫生方法提供了一种明确的替代方案。要点：非热大气压微波等离子体源以两种不同模式间接使用——气态和液态；测量腐蚀性气体PPA（等离子体处理空气）和复杂液体PTW（等离子体处理水）中短寿命和长寿命的亚硝酸盐和硝酸盐；PTW和PPA单独及联合用于不同微生物生物去污的应用。
