Dhar T K, Samanta A K, Ali E
Indian Institute of Chemical Biology, Calcutta.
Steroids. 1988 May-Jun;51(5-6):519-26. doi: 10.1016/0039-128x(88)90048-7.
A homogeneous enzyme immunoassay for estradiol estimation has been developed, which can be extended to other steroids. A new procedure for the preparation of estradiol -3-0- carboxymethyl ether by a simple one step reaction in high yield (90%) has been described. This hapten has been used for raising highly specific anti-estradiol antibody in rabbits and for preparation of enzyme conjugates. Two different enzymes, lysozyme and glucose -6- phosphate dehydrogenase have been studied for their suitability as enzyme labels. Our results indicate that lysozyme-conjugate meets the essential requirement for a practical enzyme immunoassay. The advantage of the present nonradioactive procedure is the overall simplicity, low cost and high stability of the reagents.
已开发出一种用于雌二醇测定的均相酶免疫测定法，该方法可扩展至其他甾体激素。本文描述了一种通过简单的一步反应高产率（90%）制备雌二醇 -3-0- 羧甲基醚的新方法。该半抗原已用于在兔体内产生高特异性抗雌二醇抗体以及制备酶结合物。已研究了两种不同的酶，即溶菌酶和葡萄糖 -6- 磷酸脱氢酶作为酶标记物的适用性。我们的结果表明，溶菌酶结合物满足实际酶免疫测定的基本要求。本非放射性方法的优点是整体操作简单、成本低且试剂稳定性高。
