Donà V, Deleide G, Barbieri U, Malvano R
Clin Chim Acta. 1980 Dec 8;108(2):301-7. doi: 10.1016/0009-8981(80)90017-0.
Chromatography on hydroxyapatite, including a potassium phosphate linear-gradient elution, was applied to the purification of hapten-enzyme conjugates to be used as immunoenzymatic tracers (namely, progesterone, phenobarbital, diphenylhydantoin coupled to glucose-6-phosphate dehydrogenase). The method effectively removed unreacted enzyme and separated the conjugate classes according to the number of substitutions between enzyme linking groups and hapten derivatives. The adequacy of the chromatographic procedures in improving the analytical performance of the enzymatic tracer appears to depend on direct interdependence between the degree of substitution and the immunological properties of the conjugates.
采用羟基磷灰石色谱法，包括磷酸钾线性梯度洗脱，用于纯化用作免疫酶示踪剂的半抗原 - 酶结合物（即孕酮、苯巴比妥、苯妥英与葡萄糖 -6- 磷酸脱氢酶偶联物）。该方法有效地去除了未反应的酶，并根据酶连接基团与半抗原衍生物之间的取代数分离了结合物类别。色谱程序在改善酶示踪剂分析性能方面的充分性似乎取决于取代程度与结合物免疫特性之间的直接相互依存关系。
