Department of Medical Microbiology and Immunology, University of Pécs Medical School, Hungary.
Department of Pharmacognosy, University of Pécs Medical School, Hungary.
Biomed Res Int. 2019 Jan 3;2019:2971741. doi: 10.1155/2019/2971741. eCollection 2019.
is one of the most common food-borne bacteria that causes gastrointestinal symptoms. In the present study we have investigated the molecular basis of the anti- effect of peppermint essential oil (PEO), one of the oldest EO used to treat gastrointestinal diseases. Transcriptomic, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and proteomic, two-dimensional polyacryl amid gel electrophoresis (2D-PAGE) methods have revealed that, in the presence of a sublethal concentration of PEO, the expression of several virulence-associated genes was decreased ( 0.84x; 0.79x; 0.205x; 0.08x; 0.89x; 0.25x; 4.3x) while impaired motility was revealed with a functional analysis. Scanning electron micrographs of the exposed cells showed that, unlike in the presence of other stresses, the originally curved cells straightened upon PEO exposure. Gaining insight into the molecular background of this stress response, we have revealed that in the presence of PEO dominantly exerts a general stress response that elevates the expression of general stress genes like , , (10.41x, 3.63x, and 4.77x). The most important genes , , and involved in oxidative stress responses showed however moderate transcriptional elevations (1,58x, 1,55x, and 1,85x).
是引起胃肠道症状的最常见食源性细菌之一。在本研究中，我们研究了薄荷精油（PEO）的抗作用的分子基础，PEO 是治疗胃肠道疾病最古老的精油之一。转录组学、定量逆转录聚合酶链反应（qRT-PCR）和蛋白质组学、二维聚丙烯酰胺凝胶电泳（2D-PAGE）方法表明，在亚致死浓度的 PEO 存在下，几种毒力相关基因的表达降低（0.84x；0.79x；0.205x；0.08x；0.89x；0.25x；4.3x），同时功能分析显示运动能力受损。暴露细胞的扫描电子显微镜照片显示，与其他应激源不同，原本弯曲的细胞在 PEO 暴露时变直。深入了解这种应激反应的分子背景，我们发现 PEO 主要引发一般应激反应，从而提高一般应激基因的表达，如（10.41x、3.63x 和 4.77x）。然而，参与氧化应激反应的最重要基因、和则表现出适度的转录上调（1.58x、1.55x 和 1.85x）。
