Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China.
Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, No.2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China.
Anal Bioanal Chem. 2019 Apr;411(10):2131-2140. doi: 10.1007/s00216-019-01643-2. Epub 2019 Feb 5.
Herein, we developed a sensitive and quantitative flow assay for simultaneous detection of amantadine (AMD) and chloramphenicol (CAP) in chicken samples based on different CdSe/ZnS quantum dots (QDs). In contrast to other reports, the QDs could be excited by the same excitations that lowered the requirements for the matching instruments. Under the optimal conditions, the strategy permitted sensitive detection of AMD and CAP in a linear range of 0.23 to 1.02 ng/g and 0.02 to 0.66 ng/g. The limits of detection were 0.18 ng/g and 0.016 ng/g, respectively. Moreover, the whole detection process could be completed within 20 min with no additional sophisticated instruments and complicated operations. Spiked samples were analyzed using both QD-based lateral flow immunoassay (QD-LFIA) and commercial ELISA kits with good correlation (R = 0.96). Moreover, this study laid the foundation and simplified the development of the requisite instrument. Graphical abstract ᅟ.
在此，我们开发了一种基于不同 CdSe/ZnS 量子点（QDs）的用于同时检测鸡样品中金刚烷胺（AMD）和氯霉素（CAP）的灵敏定量流动分析。与其他报道不同的是，QDs 可以被相同的激发所激发，从而降低了对匹配仪器的要求。在最佳条件下，该策略允许在 0.23 到 1.02 ng/g 和 0.02 到 0.66 ng/g 的线性范围内对 AMD 和 CAP 进行灵敏检测。检测限分别为 0.18 ng/g 和 0.016 ng/g。此外，整个检测过程可以在 20 分钟内完成，无需额外的复杂仪器和复杂操作。使用基于 QD 的侧向流动免疫分析（QD-LFIA）和商业 ELISA 试剂盒对加标样品进行分析，相关性良好（R=0.96）。此外，本研究为必要仪器的开发奠定了基础并简化了其开发。
