Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
College of Materials Science & Engineering, Sichuan University, Chengdu, 610065, China.
Mikrochim Acta. 2021 May 28;188(6):209. doi: 10.1007/s00604-021-04865-3.
Highly fluorescent hollow ZrO@CdTe nanoparticles (NPs) were synthesized efficiently via the hydrothermal method. By changing the hydrothermal time of ZrO@CdTe NP, the peaks of fluorescence spectra measured at fluorescent excitation of 330 nm were at 540 nm, 590 nm, and 640 nm, respectively. Hollow ZrO NPs have a uniform core-shell structure with the size of 178 ± 10 nm and shell of 19 ± 4 nm. The as-prepared yellow-ZrO@CdTe NPs were used to develop lateral flow assay (LFA) for the sensitive and qualitative detection of C-reactive protein (CRP). The visual limit of detection of the LFA for the CRP antigen was 1 μg/L within 20 min, which is 1000-fold lower than that of colloidal gold-based LFA. In addition, a multiplex lateral flow assay (mLFA) was developed using the as-prepared green and red-ZrO@CdTe NPs for the simultaneous, specific, sensitive, and qualitative detection of CRP and troponin T (cTnT). The visual limits of detection of CRP and cTnT in mLFA were 10 μg/L and 0.1 mg/L, respectively. The excellent performance of ZrO@CdTe NPs should facilitate their application in point-of-care technology for the detection of other biomarkers.
高效荧光中空 ZrO@CdTe 纳米粒子（NPs）通过水热法合成。通过改变 ZrO@CdTe NP 的水热时间，在 330nm 荧光激发下测量的荧光光谱的峰值分别位于 540nm、590nm 和 640nm。中空 ZrO NPs 具有均匀的核壳结构，尺寸为 178±10nm，壳层为 19±4nm。所制备的黄色-ZrO@CdTe NPs 用于开发侧向流动测定法（LFA），用于敏感和定性检测 C 反应蛋白（CRP）。LFA 对 CRP 抗原的目视检测限在 20 分钟内为 1μg/L，比基于胶体金的 LFA 低 1000 倍。此外，使用所制备的绿色和红色-ZrO@CdTe NPs 开发了多重侧向流动测定法（mLFA），用于 CRP 和肌钙蛋白 T（cTnT）的同时、特异性、敏感和定性检测。mLFA 中 CRP 和 cTnT 的目视检测限分别为 10μg/L 和 0.1mg/L。ZrO@CdTe NPs 的优异性能将有助于其在即时检测技术中用于检测其他生物标志物。
