Pelinson Luana Paula, Assmann Charles Elias, Palma Taís Vidal, da Cruz Ivana Beatrice Mânica, Pillat Micheli Mainardi, Mânica Aline, Stefanello Naiara, Weis Grazielle Castagna Cezimbra, de Oliveira Alves Audrei, de Andrade Cinthia Melazzo, Ulrich Henning, Morsch Vera Maria Melchiors, Schetinger Maria Rosa Chitolina, Bagatini Margarete Dulce
PPGBtox, CCNE, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Laboratory of Oxidative Biochemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Mol Biol Rep. 2019 Apr;46(2):2085-2092. doi: 10.1007/s11033-019-04658-1. Epub 2019 Feb 4.
Cutaneous melanoma (CM) is an extremely aggressive cancer presenting low survival and high mortality. The vast majority of patients affected by this disease does not respond or show resistance to the chemotherapeutic drugs, which makes the treatment ineffective. In this sense, the necessity for the development of new agents to assist in CM therapy is extremely important. One of the sources of great interest in this search are compounds of natural origin. Among these compounds, caffeic acid has demonstrated a broad spectrum of pharmacological activities as well as antitumor effects in some types of cancer. Therefore, the objective of this work was to investigate the possible antitumor effect of caffeic acid on the SK-Mel-28 cell line, human CM cells. Cells were cultured in flasks with culture medium containing fetal bovine serum, antibiotic, and antifungal, and maintained in ideal conditions. Cells were treated with 25 µM, 50 µM, 100 µM, 150 µM and 200 µM of caffeic acid and dacarbazine at 1 mg/mL. We verified the effect on cell viability and cell death, apoptosis, cell cycle, colony formation and gene expression of caspases. Results showed a decrease in cell viability, cell death induction by apoptosis, inhibition of colony formation, modulation of cell cycle and alterations in gene expression of caspases after caffeic acid treatment. These results suggest an antitumor effect of the compound on SK-Mel-28 cells. This study provides original information on mechanisms by which caffeic acid may play a key role in preventing tumor progression in human melanoma cells.
皮肤黑色素瘤（CM）是一种极具侵袭性的癌症，生存率低且死亡率高。绝大多数受这种疾病影响的患者对化疗药物无反应或表现出耐药性，这使得治疗无效。从这个意义上说，开发新的药物来辅助CM治疗极其重要。在这一探索中，一个备受关注的来源是天然来源的化合物。在这些化合物中，咖啡酸已显示出广泛的药理活性以及对某些类型癌症的抗肿瘤作用。因此，本研究的目的是探讨咖啡酸对人CM细胞SK-Mel-28细胞系的可能抗肿瘤作用。细胞在含有胎牛血清、抗生素和抗真菌剂的培养基的培养瓶中培养，并维持在理想条件下。细胞分别用25μM、50μM、100μM、150μM和200μM的咖啡酸以及1mg/mL的达卡巴嗪处理。我们验证了其对细胞活力、细胞死亡、凋亡、细胞周期、集落形成和半胱天冬酶基因表达的影响。结果显示，咖啡酸处理后细胞活力下降、通过凋亡诱导细胞死亡、抑制集落形成、调节细胞周期以及半胱天冬酶基因表达发生改变。这些结果表明该化合物对SK-Mel-28细胞具有抗肿瘤作用。本研究提供了关于咖啡酸可能在预防人黑色素瘤细胞肿瘤进展中起关键作用的机制的原始信息。
