Functional Experiment Center, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, Guangdong 511436, P.R. China.
Center for Reproductive Medicine, Guangdong Armed Police Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510507, P.R. China.
Mol Med Rep. 2019 Apr;19(4):2497-2502. doi: 10.3892/mmr.2019.9930. Epub 2019 Feb 4.
Phosphoglycerate mutase 1 (PGAM1) is reported to be involved in spermatogenic dysfunction. However, the association between PGAM1 and busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis remains unclear. The aim of the current study was to investigate the association between PGAM1 expression and busulfan‑induced hypospermatogenesis, and the effect of PGAM1 expression on spermatogenic cell apoptosis. PGAM1 expression was detected in mouse models of busulfan‑induced hypospermatogenesis by western blotting, reverse transcription‑quantitative polymerase chain reaction and immunohistochemistry. Then, spermatogenic cell apoptosis in mouse models of busulfan‑induced hypospermatogenesis was assessed by TUNEL assay. The effect and potential mechanism of PGAM1 downregulation on spermatogenic cells were further investigated. The results indicated that PGAM1 expression was significantly downregulated in the mouse models of busulfan‑induced hypospermatogenesis, compared with those with normal spermatogenesis (P<0.05). Furthermore, the TUNEL assay revealed that the apoptosis of spermatogenic cells was accelerated in the mouse model of busulfan‑induced hypospermatogenesis. In addition, PGAM1 knockdown promoted the apoptosis of spermatogenic cells in vitro, which was associated with the P53/Caspase 3/Caspase 6/Caspase 9 signaling pathway. In conclusion, these data indicate that PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and contributes to spermatogenic cell apoptosis by regulating the P53/Caspase 3/Caspase 6/Caspase 9 signaling pathway.
磷酸甘油酸变位酶 1（PGAM1）据报道参与精子发生功能障碍。然而，PGAM1 与白消安诱导的少精子症和生精细胞凋亡之间的关联尚不清楚。本研究旨在探讨 PGAM1 表达与白消安诱导的少精子症之间的关系，以及 PGAM1 表达对生精细胞凋亡的影响。通过 Western blot、逆转录-定量聚合酶链反应和免疫组织化学检测白消安诱导的少精子症小鼠模型中 PGAM1 的表达。然后，通过 TUNEL 检测评估白消安诱导的少精子症小鼠模型中生精细胞的凋亡。进一步研究了 PGAM1 下调对生精细胞的影响及其潜在机制。结果表明，与正常精子发生相比，白消安诱导的少精子症小鼠模型中 PGAM1 表达明显下调（P<0.05）。此外，TUNEL 检测显示，白消安诱导的少精子症小鼠模型中生精细胞的凋亡加速。此外，PGAM1 下调促进了体外生精细胞的凋亡，这与 P53/Caspase 3/Caspase 6/Caspase 9 信号通路有关。综上所述，这些数据表明，PGAM1 下调与白消安诱导的少精子症有关，并通过调节 P53/Caspase 3/Caspase 6/Caspase 9 信号通路促进生精细胞凋亡。
