磷酸甘油酸变位酶1在胶质瘤中的诊断价值及功能作用
The diagnostic value and functional roles of phosphoglycerate mutase 1 in glioma.
作者信息
Xu Zhenkuan, Gong Jie, Wang Chuanwei, Wang Yunyan, Song Yan, Xu Wenzhe, Liu Zhiguo, Liu Yuguang
机构信息
Department of Neurosurgery, Qilu Hospital of Shandong University, Brain Science Research Institute of Shandong University, Jinan, Shandong 250012, P.R. China.
Department of Neurosurgery, Shandong Provincial Hospital affiliated to Shandong University, Shandong Cancer Hospital, Shandong Provincial Institute of Cancer Prevention and Treatment, Jinan, Shandong 250012, P.R. China.
出版信息
Oncol Rep. 2016 Oct;36(4):2236-44. doi: 10.3892/or.2016.5046. Epub 2016 Aug 25.
Previous studies indicated that phosphoglycerate mutase 1 (PGAM1) is involved in many cancer types and promotes breast cancer progression. However, the role of PGAM1 in glioma remains unclear. The present study aimed to investigate the association of PGAM1 expression with glioma grade and the role of PGAM1 in proliferation, apoptosis, migration and invasion of glioma cells. The mRNA and protein expression of PGAM1 was analysed in glioma tissues and normal brain tissues. The expression of PGAM1 was examined further by immunohistochemical analysis. In addition, we inhibited the expression of PGAM1 in glioma cell line by siRNA to evaluate its role in glioma proliferation, apoptosis, migration and invasion. The mRNA and protein expression of PGAM1 was significantly greater in glioma than normal brain tissues. PGAM1 expression was associated with the WHO grade of glioma. siRNA knockdown of PGAM1 significantly inhibited glioma cell proliferation, promoted glioma cell apoptosis, induced S phase cell cycle arrest and inhibited glioma cell migration and invasion in vitro. PGAM1 may be associated with the grade of glioma and be involved in the biological behavior of glioma cells. PGAM1 might be a novel therapeutic target in glioma.
先前的研究表明，磷酸甘油酸变位酶1（PGAM1）与多种癌症类型有关，并促进乳腺癌进展。然而，PGAM1在胶质瘤中的作用仍不清楚。本研究旨在探讨PGAM1表达与胶质瘤分级的关系，以及PGAM1在胶质瘤细胞增殖、凋亡、迁移和侵袭中的作用。分析了胶质瘤组织和正常脑组织中PGAM1的mRNA和蛋白表达。通过免疫组化分析进一步检测PGAM1的表达。此外，我们通过小干扰RNA（siRNA）抑制胶质瘤细胞系中PGAM1的表达，以评估其在胶质瘤增殖、凋亡、迁移和侵袭中的作用。胶质瘤中PGAM1的mRNA和蛋白表达明显高于正常脑组织。PGAM1表达与世界卫生组织（WHO）胶质瘤分级相关。siRNA敲低PGAM1可显著抑制胶质瘤细胞增殖，促进胶质瘤细胞凋亡，诱导S期细胞周期阻滞，并在体外抑制胶质瘤细胞迁移和侵袭。PGAM1可能与胶质瘤分级有关，并参与胶质瘤细胞的生物学行为。PGAM1可能是胶质瘤的一个新的治疗靶点。
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