Department of Oncology, Zhujiang Hospital, Southern Medical University, GuangZhou City, P.R. China.
Eur Rev Med Pharmacol Sci. 2019 Jan;23(2):708-715. doi: 10.26355/eurrev_201901_16884.
Long non-coding RNA (lncRNA) is a significant member of the non-coding RNA family. New evidence has shown that it plays a pivotal role in the processes of tumor genesis and development. According to previous verification, the lncRNA Tubulin Alpha 4B (TUBA4B) is a tumor-associated molecule, but how TUBA4B expresses and functions in breast cancer is still not clear.
We conducted this study to examine what expression and biological role TUBA4B plays in breast cancer. The expression of TUBA4B was measured in breast cancer samples and cell lines. CCK8 assays and transwell assays were used for evaluating the effects of TUBA4B on breast cancer cell proliferation and invasion. Luciferase reporter assays were used for identifying the direct target of TUBA4B.
According to the results, TUBA4B was largely downregulated in breast cancer samples and cell lines. The functional analysis demonstrated that breast cancer cells proliferation and invasion could be inhibited by overexpression of TUBA4B. The results of Luciferase reporter assays indicated that TUBA4B directly targeted miR-19, which could rescue the effects of TUBA4B on breast cancer cells.
It is suggested that TUBA4B was downregulated in breast cancer and suppressed proliferation and invasion of breast cancer by targeting miR-19.
长链非编码 RNA（lncRNA）是非编码 RNA 家族的重要成员。新的证据表明，它在肿瘤发生和发展的过程中起着关键作用。根据以往的验证，微管蛋白 Alpha 4B（TUBA4B）是一种与肿瘤相关的分子，但 TUBA4B 在乳腺癌中的表达和功能如何尚不清楚。
我们进行这项研究是为了研究 TUBA4B 在乳腺癌中的表达和生物学作用。测量了乳腺癌样本和细胞系中 TUBA4B 的表达。CCK8 测定和 Transwell 测定用于评估 TUBA4B 对乳腺癌细胞增殖和侵袭的影响。荧光素酶报告测定用于鉴定 TUBA4B 的直接靶标。
结果表明，TUBA4B 在乳腺癌样本和细胞系中大量下调。功能分析表明，过表达 TUBA4B 可抑制乳腺癌细胞的增殖和侵袭。荧光素酶报告测定的结果表明，TUBA4B 可直接靶向 miR-19，从而挽救 TUBA4B 对乳腺癌细胞的作用。
提示 TUBA4B 在乳腺癌中下调，并通过靶向 miR-19 抑制乳腺癌的增殖和侵袭。
