Animal Science and Technology College, Sichuan Agricultural University, Chengdu 610000, China.
College of Agronmy, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
Bioresour Technol. 2019 May;279:166-173. doi: 10.1016/j.biortech.2019.01.107. Epub 2019 Jan 26.
The bacterial community determined via PacBio single molecule, real-time sequencing technology (SMRT) and the fermentation characteristics of Italian ryegrass (IR, 82% moisture) silage prepared with corn stover (CS) were investigated. A selected strain of Lactobacillus plantarum (L694) and a commercial inoculant stain of Lactobacillus plantarum (LP) were used as additives. Lactic acid bacteria (LAB) effectively improved silage quality. After fermentation, Lactobacillus plantarum was the dominant species in IR + LP and IR + L694 treatments, which led to higher (P < 0.05) lactic acid and lower (P < 0.05) butyric acid production. Lactobacillus plantarum, Lactobacillus hammesii, Lactobacillus brevis, and Lactobacillus coryniformis were abundantly present in IR + CS + LP and IR + CS + L694 treatments, and acetic acid contents of these were higher (P < 0.05) than those of other silages. This study demonstrated that addition of CS and LAB can change the microbial community and influence the silage fermentation of IR, and PacBio SMRT reveals more specific microbial information.
采用 PacBio 单分子实时测序技术（SMRT）测定细菌群落，并研究了添加玉米秸秆（CS）的意大利黑麦草（IR，水分 82%）青贮的发酵特性。选用一株植物乳杆菌（L694）和一株商业植物乳杆菌（LP）接种剂作为添加剂。乳酸菌（LAB）可有效改善青贮品质。发酵后，IR+LP 和 IR+L694 处理中植物乳杆菌为优势种，导致乳酸产量更高（P＜0.05），丁酸产量更低（P＜0.05）。IR+CS+LP 和 IR+CS+L694 处理中富含植物乳杆菌、汉逊德巴利酵母、短乳杆菌和棒状乳杆菌，且这些处理的乙酸含量更高（P＜0.05）。本研究表明，添加 CS 和 LAB 可以改变微生物群落，影响 IR 的青贮发酵，PacBio SMRT 揭示了更具体的微生物信息。
Zotero 插件