The potential of biogas production and effects of alfalfa silage under the synergistic influence of and pomace waste on the fermentation quality and bacterial community.

UNLABELLED

The process of ensiling serves as a dual-purpose technique, functioning as a means of preserving biomass for biogas production while serving as a biological pretreatment method with the potential for little fermentation loss. In this study, we investigate the potential of pomace (RP) and to improve the biomass preservation of biogas produced from alfalfa during anaerobic storage. Coinoculation of RP and resulted in better preservation of nutrients (biomass), with a significant increase of 47.38% within the lactic acid content and a notable decline of 40.34% in the ammonia nitrogen content relative to those in the control treatment. Moreover, coinoculation of RP and resulted in an elevated proportion of and the species dominated anaerobic fermentation. The synergistic effect of RP and continuously stimulated anaerobic fermentation, leading to 33.33% and 23.17% increases in methane production and acetate content, respectively, after 72 h of coinoculation with RP and compared to the control treatment. Overall, coinoculation of RP and offers an attractive opportunity to increase methane production from fruit waste while reducing costs and synergistically integrating with other pretreatment techniques to optimize the methane generation potential.

IMPORTANCE

Considering the increasing global energy demand and urgent environmental issues, exploring prospective resources for bioenergy production is imperative. However, the biomass of legume perennials may serve as an inexpensive and stable source of clean energy for modern society due to its wide availability and broad range of sources. In addition, the combination of RP and application increased the abundance of Lactobacillus, inhibited the growth of Kosakonia, and promoted anaerobic fermentation, which had beneficial synergistic effects on biomass retention and biogas production in alfalfa samples. Coinoculation improvements with RP and observed here are expected to reduce costs associated with CH conversion bioprocesses and increase CH production.