Li Maoya, Wang Jiachuhan, Cheng Qiming, Long Zhongfu, Chen Chao, Xie Yixiao, Lei Yao, Chen Yulian, Zhao Yuanyuan, He Xiangjiang, Yan Wei, Wang Zhijun
College of Animal Science, Guizhou University, Guiyang, Guizhou, China.
Guizhou Institute of Prataculture, Guiyang, China.
mSphere. 2025 May 27;10(5):e0105424. doi: 10.1128/msphere.01054-24. Epub 2025 May 6.
The process of ensiling serves as a dual-purpose technique, functioning as a means of preserving biomass for biogas production while serving as a biological pretreatment method with the potential for little fermentation loss. In this study, we investigate the potential of pomace (RP) and to improve the biomass preservation of biogas produced from alfalfa during anaerobic storage. Coinoculation of RP and resulted in better preservation of nutrients (biomass), with a significant increase of 47.38% within the lactic acid content and a notable decline of 40.34% in the ammonia nitrogen content relative to those in the control treatment. Moreover, coinoculation of RP and resulted in an elevated proportion of and the species dominated anaerobic fermentation. The synergistic effect of RP and continuously stimulated anaerobic fermentation, leading to 33.33% and 23.17% increases in methane production and acetate content, respectively, after 72 h of coinoculation with RP and compared to the control treatment. Overall, coinoculation of RP and offers an attractive opportunity to increase methane production from fruit waste while reducing costs and synergistically integrating with other pretreatment techniques to optimize the methane generation potential.
Considering the increasing global energy demand and urgent environmental issues, exploring prospective resources for bioenergy production is imperative. However, the biomass of legume perennials may serve as an inexpensive and stable source of clean energy for modern society due to its wide availability and broad range of sources. In addition, the combination of RP and application increased the abundance of Lactobacillus, inhibited the growth of Kosakonia, and promoted anaerobic fermentation, which had beneficial synergistic effects on biomass retention and biogas production in alfalfa samples. Coinoculation improvements with RP and observed here are expected to reduce costs associated with CH conversion bioprocesses and increase CH production.
青贮过程是一种具有双重用途的技术，既是保存生物质用于沼气生产的手段，又是一种生物预处理方法，发酵损失可能很小。在本研究中，我们研究了葡萄渣（RP）和[具体物质未明确]提高苜蓿在厌氧储存期间产生沼气的生物质保存能力的潜力。RP和[具体物质未明确]共同接种导致营养物质（生物质）得到更好的保存，乳酸含量相对于对照处理显著增加47.38%，氨氮含量显著下降40.34%。此外，RP和[具体物质未明确]共同接种导致[具体物质未明确]的比例升高，且[具体物种未明确]主导厌氧发酵。RP和[具体物质未明确]的协同作用持续刺激厌氧发酵，与对照处理相比，在与RP和[具体物质未明确]共同接种72小时后，甲烷产量和乙酸盐含量分别增加了33.33%和23.17%。总体而言，RP和[具体物质未明确]共同接种为增加水果废弃物的甲烷产量提供了一个有吸引力的机会，同时降低成本并与其他预处理技术协同整合以优化甲烷生成潜力。
考虑到全球能源需求不断增加和紧迫的环境问题，探索生物能源生产的潜在资源势在必行。然而，豆科多年生植物的生物质因其广泛可得性和丰富来源，可能成为现代社会廉价且稳定的清洁能源来源。此外，RP和[具体物质未明确]的联合应用增加了乳酸杆菌的丰度，抑制了科萨科尼亚菌的生长，并促进了厌氧发酵，这对苜蓿样品中的生物质保留和沼气生产具有有益的协同作用。此处观察到的RP和[具体物质未明确]共同接种的改进有望降低与CH转化生物过程相关的成本并增加CH产量。
