一种用于检测次氯酸根（OCl）的灵敏和选择性荧光探针及其在活细胞中的生物成像。
A sensitive and selective fluorescence probe for detection of hypochlorite (OCl) and its bioimaging in live cells.
机构信息
Faculty of Science, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China.
Department of Pharmacy, The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Shenzhen 518020, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Apr 15;213:370-374. doi: 10.1016/j.saa.2019.01.083. Epub 2019 Jan 29.
A novel indolium-based fluorescent probe (probe 1) for the recognition and detection of hypochlorite (OCl) has been explored via a double oxidation reaction mechanism. Probe 1 exhibited excellent selectivity and sensitivity for OCl over other analytes, and with a detection limit of 0.11 μM. Meanwhile, probe 1 showed fast response toward OCl in less than 3 min with obvious changes in color, which could be observed by naked eye. Moreover, fluorescence imaging experiments by using Eca109 cells were performed utilizing the new probe, demonstrating its practical applications in living cells.
一种基于吲哚的新型荧光探针（探针 1）通过双重氧化反应机制被开发用于次氯酸根（OCl）的识别和检测。探针 1 对 OCl 具有优异的选择性和灵敏度，检测限为 0.11 μM。同时，探针 1 对 OCl 的响应速度很快，不到 3 分钟即可完成，且颜色有明显变化，肉眼即可观察到。此外，还利用新探针进行了 Eca109 细胞的荧光成像实验，证明了其在活细胞中的实际应用。
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