一种用于次氯酸盐传感的线粒体靶向荧光探针及其在生物成像中的应用。
A mitochondria-targeted fluorescent probe for hypochlorite sensing and its application in bioimaging.
作者信息
Yang Qing, Zhong Xiuli, Chen Yingshuang, Yang Jing, Jin Can, Jiang Yuliang
机构信息
Nanjing Normal University, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing 210023, Jiangsu, P. R. China.
Institute of Chemical Industry of Forest Products, Jiangsu Provincial Key Laboratory of Biomass Energy and Materials, National Engineering Laboratory for Biomass Chemical Utilization, CAF, Nanjing 210042, Jiangsu, P. R. China.
出版信息
Analyst. 2020 Apr 14;145(8):3100-3105. doi: 10.1039/d0an00245c.
A coumarin-diaminomaleonitrile derivative (C-DAN) was designed and prepared. Upon addition of hypochlorite (OCl-), the fluorescence emission peak of C-DAN at 475 nm was gradually enhanced with a rapid response (within 5 s); meanwhile, the color of the solution changed from dark red to bright yellow which can be observed with the naked eye. Furthermore, owing to its advantages including high selectivity, sensitivity, and low toxicity, our C-DAN probe has been applied for detecting OCl- in living cells and zebrafish, indicating it is an ideal tool for biological applications.
设计并制备了一种香豆素-二氨基马来腈衍生物（C-DAN）。加入次氯酸盐（OCl-）后，C-DAN在475 nm处的荧光发射峰迅速增强（5 s内）；同时，溶液颜色由深红色变为亮黄色，肉眼即可观察到。此外，由于具有高选择性、高灵敏度和低毒性等优点，我们的C-DAN探针已应用于活细胞和斑马鱼中OCl-的检测，表明它是生物应用的理想工具。
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