Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Šalata12, Zagreb, Croatia.
Department of Neurosurgery, Clinical Hospital Dubrava, Av. G. Šuška 6, Zagreb, Croatia.
Cereb Cortex. 2019 Dec 17;29(11):4709-4724. doi: 10.1093/cercor/bhz004.
To uncover the ontogenesis of the human indusium griseum (IG), 28 post-mortem fetal human brains, 12-40 postconceptional weeks (PCW) of age, and 4 adult brains were analyzed immunohistochemically and compared with post-mortem magnetic resonance imaging (MRI) of 28 fetal brains (14-41 PCW). The morphogenesis of the IG occurred between 12 and 15 PCW, transforming the bilateral IG primordia into a ribbon-like cortical lamina. The histogenetic transition of sub-laminated zones into the three-layered cortical organization occurred between 15 and 35 PCW, concomitantly with rapid cell differentiation that occurred from 18 to 28 PCW and the elaboration of neuronal connectivity during the entire second half of gestation. The increasing number of total cells and neurons in the IG at 25 and 35 PCW confirmed its continued differentiation throughout this period. High-field 3.0 T post-mortem MRI enabled visualization of the IG at the mid-fetal stage using T2-weighted sequences. In conclusion, the IG had a distinct histogenetic differentiation pattern than that of the neighboring intralimbic areas of the same ontogenetic origin, and did not show any signs of regression during the fetal period or postnatally, implying a functional role of the IG in the adult brain, which is yet to be disclosed.
为了揭示人类脑岛（IG）的个体发生，我们分析了 28 例尸检胎儿人脑、12-40 孕周（PCW）和 4 例成人脑，并与 28 例胎儿脑（14-41 PCW）的死后磁共振成像（MRI）进行了比较。IG 的形态发生发生在 12 到 15 PCW 之间，将双侧 IG 原基转化为带状皮质层。亚层区向三层皮质组织的组织发生转变发生在 15 到 35 PCW 之间，伴随着快速的细胞分化，这种分化发生在 18 到 28 PCW 之间，以及在整个妊娠后半期神经元连接的形成。在 25 和 35 PCW 时 IG 中总细胞和神经元数量的增加证实了其在此期间的持续分化。高场 3.0T 死后 MRI 能够使用 T2 加权序列在中胎儿期可视化 IG。总之，IG 具有与同一个体发生起源的相邻内肢区域不同的明显组织发生分化模式，并且在胎儿期或出生后没有显示出任何退化的迹象，这意味着 IG 在成人脑中具有功能作用，而这一作用尚未被揭示。
