Plas-Roser S, Muller B, Aron C
Institute of Histology, Faculty of Medicine, Strasbourg/France.
Exp Clin Endocrinol. 1988 Dec;92(2):145-53.
The study was undertaken to examine LH, prolactin (Prl) and estradiol involvement in the control of corpus luteum function during 4-day cycle in the female rat. A potent LHRH antagonist (Antag) and bromocriptine (BRC) were used to induce LH and Prl deprivation in 4-day cyclic rats. Both drugs were administered after the occurrence of LH and Prl release on the afternoon of proestrus. Blood progesterone (P) concentration on diestrus 1 at 24:00 hr was similar in Antag, Antag + BRC and BRC-treated animals and did not differ from P values in control females. It was then concluded that neither LH nor Prl were necessary for the corpus luteum to achieve its stage of maximal activity during the night from diestrus 1 to diestrus 2. By contrast higher P values were observed on diestrus 2 at 12:00 hr in Antag-treated rats than in those given Antag + BRC, BRC or in the controls. When given on diestrus 1 evening estradiol benzoate completely prevented this effect of Antag to occur in LH and Prl deprived females. These results allow to hypothesize that LH exerts luteolytic effects during the diestrous period of the cycle and also suggest a possible involvement of estradiol in the regression phase of the corpus luteum.
本研究旨在探讨促黄体生成素（LH）、催乳素（Prl）和雌二醇在雌性大鼠4天周期中对黄体功能控制的参与情况。使用一种强效促性腺激素释放激素（LHRH）拮抗剂（Antag）和溴隐亭（BRC）诱导4天周期大鼠的LH和Prl缺乏。两种药物均在发情前期下午LH和Prl释放后给药。Antag组、Antag + BRC组和BRC处理组动物在动情间期1 24:00时的血孕酮（P）浓度相似，且与对照雌性动物的P值无差异。由此得出结论，在从动情间期1到动情间期2的夜间，黄体达到最大活性阶段既不需要LH也不需要Prl。相比之下，在动情间期2 中午12:00时，Antag处理组大鼠的P值高于给予Antag + BRC组、BRC组或对照组大鼠。在动情间期1晚上给予苯甲酸雌二醇可完全阻止Antag对LH和Prl缺乏雌性动物的这种作用。这些结果使我们能够推测，LH在周期的动情期发挥溶黄体作用，并且还提示雌二醇可能参与黄体的退化阶段。
