酶法在 sn-2 位制备富含花生四烯酸和棕榈酸的结构三酰基甘油，用于婴儿配方食品。
Enzymatic preparation of structured triacylglycerols with arachidonic and palmitic acids at the sn-2 position for infant formula use.
机构信息
National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, PR China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), 11 Fucheng Road, Beijing 100048, China.
National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, PR China.
出版信息
Food Chem. 2019 Jun 15;283:331-337. doi: 10.1016/j.foodchem.2019.01.057. Epub 2019 Jan 17.
Structured TAGs with palmitic acid and polyunsaturated fatty acid (PUFA) at the sn-2 position have various health benefits for infants. In this study, we first compared two enzymatic routes for preparation of the structured TAGs. Results showed that the one-pot and two-step syntheses led to 37.6% and 55.4% oleic acid incorporation, respectively, after 10 h and reaction route had little effect on the sn-2 fatty acid composition. Subsequently, reaction variables of the two-step synthesis were optimized. Under the optimal conditions, 53.5% oleic acid was incorporated into the structured TAGs after 6-h acidolysis. Major fatty acids at the sn-2 position were palmitic acid (68.7%), ARA (9.8%) and oleic acid (7.9%). This is the first study reporting a two-step enzymatic method for structured TAGs preparation. Compared to the one-pot synthesis, current method significantly improves the efficiency of the acidolysis by product inhibition elimination. The synthetic TAGs have potential use in infant formulas.
具有棕榈酸和多不饱和脂肪酸（PUFA）的结构化 TAG 在 sn-2 位置具有各种有益于婴儿的健康益处。在这项研究中，我们首先比较了两种酶法合成结构化 TAG 的途径。结果表明，一锅法和两步法合成分别在 10 小时后导致 37.6%和 55.4%的油酸掺入，反应途径对 sn-2 脂肪酸组成的影响很小。随后，优化了两步法合成的反应变量。在最佳条件下，6 小时酸解后，53.5%的油酸被掺入结构化 TAG 中。sn-2 位的主要脂肪酸为棕榈酸（68.7%）、ARA（9.8%）和油酸（7.9%）。这是首次报道两步酶法合成结构化 TAG 的研究。与一锅法合成相比，目前的方法通过消除产物抑制显著提高了酸解效率。合成的 TAG 有可能用于婴儿配方奶粉。
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