Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan 87317-51167, Islamic Republic of Iran.
Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan 87317-51167, Islamic Republic of Iran.
Food Chem. 2019 Jun 15;283:68-72. doi: 10.1016/j.foodchem.2018.12.132. Epub 2019 Jan 14.
Multivariate curve resolution-alternating least squares (MCR-ALS) assisted with electrochemical techniques was applied for simultaneous determination of Sudan II and III at a surface of zinc oxide nanoparticles (ZnONPs) modified carbon paste electrode. Characterization of the ZnONPs and the nanostructured modified electrode was performed by X-ray diffraction (XRD), scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. Response surface methodology using central composite rotatable design was employed to design of experiments and achieving to optimum values of variables. For simultaneous determination of Sudan II and III, electrochemical second order data were obtained by changing of pulse height in differential pulse voltammetry technique. MCR-ALS was performed on the data to obtain the decomposition peaks of analytes. Then, the detection limits were obtained 1.87 and 2.62 nM for Sudan II and III, respectively. Finally, this method used for the analysis of the two analytes in real samples.
多变量曲线分辨-交替最小二乘法 (MCR-ALS) 与电化学技术结合，用于在氧化锌纳米粒子 (ZnONPs) 修饰碳糊电极表面同时测定苏丹 II 和 III。通过 X 射线衍射 (XRD)、扫描电子显微镜 (SEM)、电化学阻抗谱 (EIS) 和循环伏安法 (CV) 技术对 ZnONPs 和纳米结构修饰电极进行了表征。响应面法采用中心复合旋转设计进行实验设计，以达到变量的最佳值。为了同时测定苏丹 II 和 III，通过改变差分脉冲伏安法技术中的脉冲高度获得电化学二阶数据。对数据进行 MCR-ALS 处理，以获得分析物的分解峰。然后，苏丹 II 和 III 的检测限分别为 1.87 和 2.62 nM。最后，该方法用于实际样品中两种分析物的分析。
