State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, China.
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
Sci Rep. 2019 Feb 5;9(1):1452. doi: 10.1038/s41598-018-36529-3.
Cellulose synthase-like D (CSLD) family was characterized for their expression and functions in Populus trichocarpa. Ten members, PtrCslD1-10, were identified in the P. trichocarpa genome, and they belong to 4 clades by phylogenetic tree analysis. qRT-PCR and promoter:GUS assays in Arabidopsis and P. trichocarpa displayed divergent expression patterns of these 10 PtrCSLD genes in root hairs, root tips, leaves, vascular tissues, xylem and flowers. Among PtrCslD2, PtrCslD4, PtrCslD5, PtrCslD6, and PtrCslD8 that all exhibited expression in root hairs, only PtrCslD5 could restore the root hairless phenotype of the atcsld3 mutant, demonstrating that PtrCslD5 is the functional ortholog of AtCslD3 for root hair formation. Our results suggest more possible functions for other PtrCslD genes in poplar.
纤维素合酶类似蛋白 D（CSLD）家族因其在杨树中的表达和功能而被研究。在杨树木耳基因组中鉴定出 10 个成员（PtrCslD1-10），通过系统发育树分析，它们属于 4 个分支。在拟南芥和杨树木耳中进行的 qRT-PCR 和启动子：GUS 分析显示，这 10 个 PtrCSLD 基因在根毛、根尖、叶片、维管束组织、木质部和花中的表达模式存在差异。在所有在根毛中表达的 PtrCslD2、PtrCslD4、PtrCslD5、PtrCslD6 和 PtrCslD8 中，只有 PtrCslD5 能够恢复 atcsld3 突变体的无根毛表型，表明 PtrCslD5 是 AtCslD3 形成根毛的功能同源物。我们的结果表明，其他 PtrCslD 基因在杨树中可能具有更多的功能。
