State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin, 150040, PR China; School of Forestry, Northeast Forestry University, Harbin, 150040, China.
Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin, 150040, China; College of Life Science, Northeast Forestry University, Harbin, 150040, China.
Gene Expr Patterns. 2020 Dec;38:119142. doi: 10.1016/j.gep.2020.119142. Epub 2020 Sep 6.
Reversible glycosylation polypeptide (RGP) is a type of plant-specific protein, primarily involved in the biosynthesis of cell wall polysaccharides, which in turn changes the shape of the cell walls and affects the wood properties of plants. Poplar is a major industrial timber species, and the RGP gene has not been studied. This study uses bioinformatics methods to predict physical and chemical characters such as molecular weight, isoelectric point, and hydrophilicity; and fluorescent quantitative method to determine the effect of different forms of nitrogen on the transcription level of the gene family. The results showed that there are six RGP homologous genes in the Populus trichocarpa genome, which were distributed on the six chromosomes of P. trichocarpa. The family members have a simple gene structure and contain four exons and introns. Phylogenetic tree analysis showed that RGP genes all belong to Class I in P. trichocarpa. Tissue-specific expression analysis showed that PtRGP1 and PtRGP2 were highly expressed in the stems, PtRGP4 and PtRGP5 were highly expressed in the upper leaves, PtRGR3 and PtRGR6 were expressed in stems and internodes, but the relative expression is not high. Quantitative real-time RT-PCR (qRT-PCR) analyses revealed that PtRGP3 and 6 were up-regulated in the upper stem in response to the low ammonium and high nitrate treatments. The influence of nitrogen on the expression of PtRGP3 and 6 genes may affect the formation of the plant secondary cell wall. This study lays a foundation for further study on the function of RGP genes in P. trichocarpa.
可逆糖基化多肽（RGP）是一种植物特异性蛋白，主要参与细胞壁多糖的生物合成，从而改变细胞壁的形状并影响植物的木材特性。杨树是一种主要的工业木材树种，而 RGP 基因尚未得到研究。本研究利用生物信息学方法预测分子量、等电点和亲水性等理化特性；并采用荧光定量方法确定不同形式氮对基因家族转录水平的影响。结果表明，在毛白杨基因组中存在 6 个 RGP 同源基因，它们分布在毛白杨的 6 条染色体上。家族成员具有简单的基因结构，包含 4 个外显子和内含子。系统进化树分析表明，RGP 基因在毛白杨中均属于 I 类。组织特异性表达分析表明，PtRGP1 和 PtRGP2 在茎中高表达，PtRGP4 和 PtRGP5 在顶叶中高表达，PtRGR3 和 PtRGR6 在茎和节间表达，但相对表达量不高。定量实时 RT-PCR（qRT-PCR）分析显示，PtRGP3 和 6 在上部茎中对低铵和高硝态氮处理上调。氮对 PtRGP3 和 6 基因表达的影响可能影响植物次生细胞壁的形成。本研究为进一步研究 RGP 基因在毛白杨中的功能奠定了基础。
