Department of Neurosurgery, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, 510120, China.
Guangdong Provincial Key Laboratory of Medical Biomechanics, Department of Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China.
J Biomed Mater Res A. 2019 Jun;107(6):1264-1272. doi: 10.1002/jbm.a.36636. Epub 2019 Feb 22.
Introducing or grafting molecules onto biomaterial surfaces to regulate muscle cell destination via biophysical cues is one of the important steps for biomaterial design in muscle tissue engineering. Therefore, it is important to understand the interaction between myoblasts and myofibers with substrates modified by biomimetic layer with different thicknesses. In this study, we used a surface-induced atom transfer radical polymerization method to synthetize and graft poly-phenoxyethyl methacrylate (PHEMA) brushes having different lengths on the glass substrates. C2C12 myoblasts were seeded on the PHEMA brushes and differentiated using horse serum, for analyzing the sensibility of muscle cells to feel environment changing, and further investigating whether the depths of grafting layer on the biomaterial surface are important factors in regulating muscle cell behaviors. Our results demonstrated that on the thicker PHEMA brushes surface (200 and 450 nm), C2C12 myoblasts showed a better survival and proliferation and were favorable for cell fusion and myotube formation. Furthermore, myofibers survived on the thicker brushes were more functional and upregulated cytoskeleton proteins (tubulin, vimentin, and vinculin) and FAK levels, and enhanced the expression levels for mechanical stress molecules (HGF, NOS-1, and c-Met). These results suggest that grafting thickness of PHEMA layer on the substrate led to the myoblasts/myofiber behavior change, which would be valuable for the design and preparation of the modified layer on muscle tissue engineering scaffolds. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1264-1272, 2019.
在生物材料表面引入或嫁接分子，通过生物物理线索调节肌肉细胞的去向，是肌肉组织工程中生物材料设计的重要步骤之一。因此，了解具有不同厚度仿生层修饰的基底上的成肌细胞与肌纤维之间的相互作用非常重要。在这项研究中，我们使用表面诱导原子转移自由基聚合方法，在玻璃基底上合成并嫁接不同长度的聚-苯氧乙基甲基丙烯酸酯（PHEMA）刷。将 C2C12 成肌细胞接种在 PHEMA 刷上，并用马血清进行分化，用于分析肌肉细胞对环境变化的敏感性，进一步研究生物材料表面嫁接层的深度是否是调节肌肉细胞行为的重要因素。我们的结果表明，在较厚的 PHEMA 刷表面（200nm 和 450nm）上，C2C12 成肌细胞表现出更好的存活和增殖能力，有利于细胞融合和肌管形成。此外，在较厚的刷上存活的肌纤维更具功能性，并上调细胞骨架蛋白（微管蛋白、波形蛋白和 vinculin）和 FAK 水平，并增强机械应激分子（HGF、NOS-1 和 c-Met）的表达水平。这些结果表明，基底上 PHEMA 层的嫁接厚度导致成肌细胞/肌纤维行为发生变化，这对于肌肉组织工程支架改性层的设计和制备具有重要意义。 © 2019 Wiley Periodicals, Inc. J 生物材料 Res 部分 A: 107A: 1264-1272, 2019.
