Department of Food Science and Technology, Federal University of Santa Catarina, Rodovia Admar Gonzaga 1346, 88034-001 Florianópolis, SC, Brazil; Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
Food Chem. 2019 Mar 1;275:123-134. doi: 10.1016/j.foodchem.2018.09.086. Epub 2018 Sep 14.
We formulated and characterised two alginate blends for the encapsulation of stevia extract (SE) via ionic gelation through an extrusion technique. Calcium chloride in SE and calcium chloride solutions were assessed as crosslinkers to overcome phenolic losses by diffusion and increase encapsulation efficiency (EE). Regardless of the blend, all stevia-loaded beads exhibited high EE (62.7-101.0%). The size of the beads decreased as EE increased. Fourier transform infrared analysis showed increased hydrogen bonding between SE and alginates, confirming the successful incorporation of SE within the matrix. Untargeted metabolomics profiling identified 479 free and encapsulated polyphenolic compounds. Flavonoids (catechin and luteolin equivalents) were predominant in SE whereas tyrosols and 5-pentadecylresorcinol equivalents were predominant in all bead formulations. Three-common discriminant compounds were exclusive to each blend and were inversely affected by the crosslinking conditions. Both alginate blends have been shown to be feasible as carrier systems of stevia extracts independent of crosslinking conditions.
我们通过离子凝胶化挤出技术，使用两种藻酸盐混合物对甜菊提取物（SE）进行包封。通过评估 SE 中的氯化钙和氯化钙溶液作为交联剂，可以克服扩散引起的酚类损失，提高包封效率（EE）。无论使用哪种混合物，所有载有甜菊的珠粒都表现出高 EE（62.7-101.0%）。珠粒的大小随着 EE 的增加而减小。傅里叶变换红外分析显示 SE 和藻酸盐之间氢键增加，证实 SE 已成功地掺入基质中。非靶向代谢组学分析鉴定出 479 种游离和包封的多酚化合物。SE 中以类黄酮（儿茶素和木樨草素当量）为主，而 tyrosols 和 5-十五烷基间苯二酚当量则在所有珠粒配方中占主导地位。三种共同的判别化合物是每种混合物特有的，并且受交联条件的影响相反。这两种藻酸盐混合物均被证明是甜菊提取物的可行载体系统，与交联条件无关。
