Toprakçı Gizem, Toprakçı İrem, Şahin Selin
Chemical Engineering Department, Faculty of Engineering, Istanbul University-Cerrahpasa, Avcilar, 34320 Istanbul, Türkiye.
Gels. 2025 Feb 27;11(3):172. doi: 10.3390/gels11030172.
Medical and aromatic plant extracts are often very sensitive to environmental, gastrointestinal, and processing conditions despite their health benefits. Therefore, they can be rapidly inactivated. Microencapsulation is used to overcome such challenges. In this study, phenolic antioxidants from rosemary ( L.) were encapsulated in alginate beads by means of ionic gelation. A Box-Behnken design with response surface methodology (BBD-RSM) was used with three numeric factors (calcium chloride concentration, alginate concentration, and hardening time) to achieve the best formulation in terms of encapsulation efficiency, antioxidant activity, and morphological characteristics. Generally, the sodium alginate concentration of the microbeads was the most critical factor ( < 0.0001) for the quality of the products. The optimal encapsulation conditions were accessed using concentrations with almost 6% calcium chloride and 2% alginate, and a time of 10 min for bead hardening in order to obtain the highest responses (30.01% encapsulation efficiency, 7.55 mg-TEAC/g-DM of antioxidant activity value as measured by the DPPH method, a sphericity factor of 0.05, and a roundness of 0.78). At the optimum point, the microbeads were determined to be spherical in shape, and the bulk density value was measured as 0.34 ± 0.01 g/mL.
尽管药用和芳香植物提取物具有健康益处，但它们通常对环境、胃肠道和加工条件非常敏感。因此，它们可能会迅速失活。微胶囊化用于克服此类挑战。在本研究中，迷迭香叶中的酚类抗氧化剂通过离子凝胶法包封在海藻酸钠珠中。采用响应面法的Box-Behnken设计（BBD-RSM），以三个数值因素（氯化钙浓度、海藻酸钠浓度和硬化时间）来实现包封效率、抗氧化活性和形态特征方面的最佳配方。一般来说，微珠的海藻酸钠浓度是影响产品质量的最关键因素（<0.0001）。使用约6%的氯化钙和2%的海藻酸钠浓度以及10分钟的珠硬化时间来获得最佳包封条件，以获得最高响应值（包封效率30.01%，通过DPPH法测得的抗氧化活性值为7.55 mg-TEAC/g-DM，球形度因子为0.05，圆度为0.78）。在最佳点，确定微珠为球形，堆积密度值测量为0.34±0.01 g/mL。
