Australian Centre for Research on Separation Science, School of Natural Sciences, University of Tasmania, Hobart, TAS 7001, Australia; Australian Centre for Research on Separation Science, School of Chemistry, Monash University, Wellington Road, Clayton, VIC 3800, Australia.
Australian Centre for Research on Separation Science, School of Chemistry, Monash University, Wellington Road, Clayton, VIC 3800, Australia; Present address: School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
Food Chem. 2019 Mar 1;275:15-23. doi: 10.1016/j.foodchem.2018.09.082. Epub 2018 Sep 14.
This study investigated the volatile phytochemical diversity of 30 samples obtained from experimental hybrid and commercial H. lupulus L. plants. Essential oils distilled from these samples were analysed by high resolution gas chromatography coupled with accurate mass time-of-flight mass spectrometry (GC-accTOFMS). A total of 58 secondary metabolites, mainly comprising 18 esters, 6 monoterpene hydrocarbons, 2 oxygenated monoterpenes, 20 sesquiterpene hydrocarbons, 7 oxygenated sesquiterpenes, and 4 ketones, were positively or tentatively identified. A total of 24 metabolites were detected in all samples, but commercial cultivars (selected for brewing performance) had fewer compounds identified compared to experimental genotypes. Chemometrics analyses enabled distinct differentiation of experimental hybrids from commercial cultivars, discussed in terms of the different classes of compounds present in different genotypes. Differences among the mono- and sesquiterpenoids, appear to be related to either: i) the genetic origin of the plants; or ii) the processes of bioaccumulation of the identified secondary metabolites.
本研究调查了 30 个实验杂交和商业啤酒花（Humulus lupulus L.）植物样本的挥发性植物化学多样性。从这些样本中蒸馏出的精油通过高分辨率气相色谱与精确质量飞行时间质谱联用（GC-accTOFMS）进行分析。总共鉴定出 58 种次生代谢物，主要包括 18 种酯类、6 种单萜烃类、2 种含氧单萜类、20 种倍半萜烃类、7 种含氧倍半萜类和 4 种酮类。所有样品中均检测到 24 种代谢物，但与实验基因型相比，商业品种（为酿造性能选择）鉴定出的化合物较少。化学计量学分析能够明显区分实验杂交种和商业品种，这与不同基因型中存在的不同类别的化合物有关。单萜和倍半萜类化合物之间的差异似乎与以下两个方面有关：i）植物的遗传起源；或 ii）鉴定出的次生代谢物的生物积累过程。
