Holanda Gustavo Moraes, Casseb Samir Mansour Morais, Quaresma Juarez Antônio Simões, Vasconcelos Pedro Fernando Costa, Cruz Ana Cecília Ribeiro
Instituto Evandro Chagas, Rodovia BR-316 Km7, Levilândia, Ananindeua, PA, CEP 67030-000, Brazil.
Arch Virol. 2019 Apr;164(4):1187-1192. doi: 10.1007/s00705-019-04171-7. Epub 2019 Feb 6.
Yellow fever virus (YFV) penetrates the skin through the bite of a vector mosquito and spreads to various organs, mainly the liver, where it causes lesions and induces necrosis and apoptosis. We evaluated the mRNA expression of various cytokines and the activation of caspases in HepG2 cells infected with YFV. We observed that interferon-α (IFN-α) expression decreased and IFN-β, transforming growth factor (TGF)-β IIIR, interleukin (IL)-6, and IL-8 expression increased in cells infected with genotype 1. In contrast, TNF-α expression increased in cells infected with genotype 2 but not with genotype 1. This provides insights into the role of cytokine regulation in yellow fever.
黄热病病毒（YFV）通过媒介蚊子叮咬穿透皮肤，并扩散到各个器官，主要是肝脏，在那里它会引发病变并诱导坏死和凋亡。我们评估了感染YFV的HepG2细胞中各种细胞因子的mRNA表达以及半胱天冬酶的激活情况。我们观察到，感染1型病毒的细胞中，干扰素-α（IFN-α）表达下降，而IFN-β、转化生长因子（TGF）-βIIIR、白细胞介素（IL）-6和IL-8表达增加。相比之下，感染2型病毒的细胞中肿瘤坏死因子-α（TNF-α）表达增加，而感染1型病毒的细胞中则没有增加。这为细胞因子调节在黄热病中的作用提供了见解。
