Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM).

The mitochondrial membrane potential (Δψ) drives the generation of ATP by mitochondria. Interestingly, Δψ is higher in many cancer cells comparted to healthy noncancerous cell types, providing a unique metabolic marker. This feature has also been exploited for therapeutic use by utilizing drugs that specifically accumulate in the mitochondria of cancer cells with high Δψ. As such, the assessment of Δψ can provide very useful information as to the metabolic state of a cancer cell, as well as its potential for malignancy. In addition, the measurement of Δψ can also be used to test the ability of novel anticancer therapies to disrupt mitochondrial metabolism and cause cell death.Here, we outline two methods for assessing Δψ in cancer cells using confocal microscopy and the potentiometric fluorescent dye tetramethylrhodamine methyl ester (TMRM). In the first protocol, we describe a technique to quantitatively measure Δψ, which can be used to compare Δψ between different cell types. In the second protocol, we describe a technique for assessing changes to Δψ over time, which can be used to determine the effectiveness of different therapeutic compounds or drugs in modulating mitochondrial function.