Morales Elisa, Chen Jun, Greathouse K Leigh
Robbins College of Health and Human Sciences, Baylor University, Waco, TX, USA.
Division of Biomedical Statistics and Informatics, Department of Health Sciences Research, Mayo Clinic, Rochester, Minnesota, USA.
Methods Mol Biol. 2019;1928:299-335. doi: 10.1007/978-1-4939-9027-6_16.
Gut microbial composition has shown to be associated with obesity, diabetes mellitus, inflammatory bowel disease, colitis, autoimmune disorders, and cancer, among other diseases. Microbiome research has significantly evolved through the years and continues to advance as we develop new and better strategies to more accurately measure its composition and function. Careful selection of study design, inclusion and exclusion criteria of participants, and methodology are paramount to accurately analyze microbial structure. Here we present the most up-to-date available information on methods for gut microbial collection and analysis.
肠道微生物组成已被证明与肥胖、糖尿病、炎症性肠病、结肠炎、自身免疫性疾病和癌症等多种疾病有关。多年来，微生物组研究有了显著进展，并且随着我们开发更新更好的策略以更准确地测量其组成和功能，这一研究仍在不断推进。仔细选择研究设计、参与者的纳入和排除标准以及方法对于准确分析微生物结构至关重要。在此，我们展示了关于肠道微生物收集和分析方法的最新可用信息。
