González Mariela, García María E, Slanis Alberto, Bonini Ana, Fiedler Stephanie, Fariña Laura, Dellacassa Eduardo, Condurso Concetta, Lorenzo Daniel, Russo Marcos, Tereschuk María L
Departamento de Ingeniería de Procesos y Gestión Industrial, Facultad de Ciencias Exactas y Tecnología, Universidad Nacional de Tucumán, San Miguel de Tucumán, 4000, República Argentina.
Laboratorio de Palinología, Fundación Miguel Lillo, Miguel Lillo 251, San Miguel de Tucumán, 4000, República Argentina.
Chem Biodivers. 2019 May;16(5):e1800442. doi: 10.1002/cbdv.201800442. Epub 2019 Apr 4.
Propolis samples from north-west Argentina (Amaicha del Valle, Tucumán) were evaluated by palynology, FT-IR spectra, and RP-HPTLC. In addition, the volatile fraction was studied by HS-SPME-GC/MS. The botanical species most visited by Apis mellifera L. near the apiaries were collected and their RP-HPTLC extracts profiles were compared with propolis samples. In addition, GC/MS was performed for volatile compounds from Zuccagnia punctata Cav. (Fabaceae). FT-IR spectra and RP-HPTLC fingerprints of propolis samples showed similar profiles. In RP-HPTLC analyses, only Z. punctata presented a similar fingerprint to Amaicha propolis. The major volatile compounds present in both were trans-linalool oxide (furanoid), 6-camphenone, linalool, trans-pinocarveol, p-cymen-8-ol, and 2,3,6-trimethylbenzaldehyde. Potential variations for the Amaicha del Valle propolis volatile fraction as consequence of propolis sample preparation were demonstrated.
对来自阿根廷西北部（图库曼省阿迈查德尔瓦莱）的蜂胶样本进行了孢粉学、傅里叶变换红外光谱（FT-IR）和反相高效薄层色谱（RP-HPTLC）分析。此外，采用顶空固相微萃取-气相色谱/质谱联用（HS-SPME-GC/MS）技术研究了其挥发性成分。采集了蜂场附近意大利蜜蜂最常采蜜的植物种类，并将其RP-HPTLC提取物谱与蜂胶样本进行比较。此外，还对豆科植物斑叶苏卡尼亚（Zuccagnia punctata Cav.）的挥发性化合物进行了气相色谱/质谱分析。蜂胶样本的傅里叶变换红外光谱和反相高效薄层色谱指纹图谱显示出相似的特征。在反相高效薄层色谱分析中，只有斑叶苏卡尼亚呈现出与阿迈查蜂胶相似的指纹图谱。两者中主要的挥发性化合物为反式芳樟醇氧化物（呋喃型）、6-蒈酮、芳樟醇、反式松油醇、对伞花烃-8-醇和2,3,6-三甲基苯甲醛。研究表明，由于蜂胶样本制备的原因，阿迈查德尔瓦莱蜂胶挥发性成分可能存在差异。
