Instituto de Biotecnología, Universidad Nacional de San Juan, Avenida Libertador General San Martin 1109 (O), 5400 San Juan, Argentina.
J Agric Food Chem. 2010 Jan 13;58(1):194-201. doi: 10.1021/jf902991t.
This paper reports the in vitro antifungal activity of propolis extracts from the province of Tucuman (Argentina) as well as the identification of their main antifungal compounds and botanical origin. The antifungal activity was determined by the microdilution technique, using reference microorganisms and clinical isolates. All dermatophytes and yeasts tested were strongly inhibited by different propolis extracts (MICs between 16 and 125 microg mL(-1)). The most susceptible species were Microsporum gypseum, Trichophyton mentagrophytes, and Trichophyton rubrum. The main bioactive compounds were 2',4'-dihydroxy-3'-methoxychalcone 2 and 2',4'-dihydroxychalcone 3. Both displayed strong activity against clinical isolates of T. rubrum and T. mentagrophytes (MICs and MFCs between 1.9 and 2.9 microg mL(-1)). Additionally, galangin 5, pinocembrin 6, and 7-hydroxy-8-methoxyflavanone 9 were isolated from propolis samples and Zuccagnia punctata exudates, showing moderate antifungal activity. This is the first study matching the chemical profile of Z. punctata Cav. exudates with their corresponding propolis, giving strong evidence on the botanical origin of the studied propolis.
本文报道了阿根廷图库曼省蜂胶提取物的体外抗真菌活性，以及其主要抗真菌化合物和植物来源的鉴定。采用微量稀释法，使用参考微生物和临床分离株来测定抗真菌活性。所有测试的皮肤癣菌和酵母菌均被不同的蜂胶提取物强烈抑制（MIC 在 16 到 125 微克/毫升之间）。最敏感的物种是石膏样小孢子菌、须癣毛癣菌和红色毛癣菌。主要的生物活性化合物是 2',4'-二羟基-3'-甲氧基查耳酮 2 和 2',4'-二羟基查耳酮 3。两者对红色毛癣菌和须癣毛癣菌的临床分离株均显示出很强的活性（MIC 和 MFC 在 1.9 到 2.9 微克/毫升之间）。此外，从蜂胶样品和 Zuccagnia punctata 渗出物中分离出了白杨素 5、松属素 6 和 7-羟基-8-甲氧基黄烷酮 9，显示出中等的抗真菌活性。这是首次将 Zuccagnia punctata Cav.渗出物的化学特征与其相应的蜂胶相匹配的研究，为所研究的蜂胶的植物来源提供了有力的证据。
