Hamada Hiroki, Okada Shouta, Shimoda Kei, Uesugi Daisuke, Hamada Hatsuyuki
Nat Prod Commun. 2016 Aug;11(8):1121-1122.
The optical resolution of racemic compounds by stereoselective glucosylation was investigated using plant glucosyltransferase from Phytolacca americana expressed in recombinant Escherichia coli. The glucosyltransferase glucosylated chemoselectively the phenolic hydroxyl group of phenol compounds. The (R)-stereoselective glucosylation of (RS)-denopamine by glucosyltransferase occurred to give (R)-denopamine β-D-glucoside.
利用在重组大肠杆菌中表达的来自美洲商陆的植物糖基转移酶，研究了通过立体选择性糖基化拆分外消旋化合物。该糖基转移酶对酚类化合物的酚羟基进行化学选择性糖基化。糖基转移酶对（RS）-去甲肾上腺素进行（R）-立体选择性糖基化，生成（R）-去甲肾上腺素β-D-葡萄糖苷。
