Wang Shuqin, Fu Liying, Du Wenhui, Hu Jun, Zha Yongsheng, Wang Peiguang
Department of Dermatology, 1st Affiliated Hospital, Anhui Medical University, Hefei, China.
Department of Dermatology, the Anqing Municipal Hospital, Anqing, China.
An Bras Dermatol. 2019 Jan-Feb;94(1):52-55. doi: 10.1590/abd1806-4841.20197494.
Pityriasis rosea is a common papulosquamous disorder. However, its etiology and pathogenesis remain unclear.
We investigate the types of inflammatory cells infiltrating the lesional skin of pityriasis rosea and demonstrate whether T-cell-mediated immunity is involved in the pathogenesis of this condition or not.
The biopsies were taken from the lesional skin of 35 cases of patients diagnosed with pityriasis rosea. The specimens were prepared in paraffin sections, then submitted to routine immunohistochemistry procedures using monoclonal antibodies directed against CD3, CD4, CD8, CD20 and CD45RO and horseradish peroxidase-labeled goat anti-human antibodies. The positive sections were determined by the ratio and staining intensity of positive inflammatory cells.
The mean score of positive CD3, CD4, CD8, and CD45RO staining was respectively 3.74±3.88, 5.67±4.40, 2.94±3.42 and 7.68±4.33 in these pityriasis rosea patients (P<0.001). The percentage of positive staining was 54.29% (19/35), 69.7% (23/33), 40% (14/35) and 79.41% (27/34) (P<0.05). However, the staining of CD20 was negative in all samples. The mean score of CD3 staining in patients with time for remission ≤60 days (4.90±4.21) was higher than that in patients with time for remission >60 days (2.00±2.5) (P<0.05), whereas no statistical difference in the mean score of CD4, CD8 and CD45RO staining was observed. study liMitations: The sample size and the selected monoclonal antibody are limited, so the results reflect only part of the cellular immunity in the pathogenesis of pityriasis rosea.
Our findings support a predominantly T-cell mediated immunity in the development of pityriasis rosea.
玫瑰糠疹是一种常见的丘疹鳞屑性皮肤病。然而，其病因和发病机制仍不清楚。
我们研究浸润玫瑰糠疹皮损的炎症细胞类型，并证明T细胞介导的免疫是否参与该病的发病机制。
对35例诊断为玫瑰糠疹的患者的皮损进行活检。标本制成石蜡切片，然后使用抗CD3、CD4、CD8、CD20和CD45RO的单克隆抗体及辣根过氧化物酶标记的山羊抗人抗体进行常规免疫组织化学程序。通过阳性炎症细胞的比例和染色强度确定阳性切片。
这些玫瑰糠疹患者中，CD3、CD4、CD8和CD45RO染色阳性的平均得分分别为3.74±3.88、5.67±4.40、2.94±3.42和7.68±4.33（P<0.001）。阳性染色百分比分别为54.29%（19/35）、69.7%（23/33）、40%（14/35）和79.41%（27/34）（P<0.05）。然而，所有样本中CD20染色均为阴性。缓解时间≤60天的患者CD3染色平均得分（4.90±4.21）高于缓解时间>60天的患者（2.00±2.5）（P<0.05），而CD4、CD8和CD45RO染色平均得分无统计学差异。研究局限性：样本量和所选单克隆抗体有限，因此结果仅反映玫瑰糠疹发病机制中细胞免疫的一部分。
我们的研究结果支持玫瑰糠疹的发病主要由T细胞介导的免疫。
