Addy Hardian S, Askora Ahmed, Kawasaki Takeru, Fujie Makoto, Yamada Takashi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan.
Plant Dis. 2012 Aug;96(8):1204-1209. doi: 10.1094/PDIS-12-11-1023-RE.
The wide host range of Ralstonia solanacearum, causal agent of bacterial wilt, and its ability to survive for long periods in the environment restrict the effectiveness of cultural and chemical control measures. The use of phages for disease control is a fast-expanding trend of plant protection with great potential to replace chemical measures. The filamentous phage ϕRSM3 that infects R. solanacearum strains and inactivates virulence on plants is a potential agent for controlling bacterial wilt in tomato. We demonstrated that inoculation of ϕRSM3-infected cells into tomato plants did not cause bacterial wilt. Instead, ϕRSM3-infected cells enhanced the expression of pathogenesis-related (PR) genes, including PR-1a, PR-2b, and PR7, in tomato plants. Moreover, pretreatment with ϕRSM-infected cells protect tomato plants from infection by virulent R. solanacearum strains. The effective dose of ϕRSM3-infected cells for disease prevention was determined to be approximately 10 CFU/ml. Because the ϕRSM3-infected cells can grow and continue to produce infectious phage particles under appropriate conditions, ϕRSM phages may serve as an efficient tool to control bacterial wilt in crops.
青枯雷尔氏菌是青枯病的病原菌，其寄主范围广泛，且能在环境中长时间存活，这限制了栽培和化学防治措施的效果。利用噬菌体进行病害防治是植物保护领域快速发展的趋势，具有很大的潜力来取代化学防治措施。丝状噬菌体ϕRSM3可感染青枯雷尔氏菌菌株并使植物的毒力失活，是控制番茄青枯病的潜在因子。我们证明，将感染ϕRSM3的细胞接种到番茄植株中不会引发青枯病。相反，感染ϕRSM3的细胞会增强番茄植株中病程相关（PR）基因的表达，包括PR-1a、PR-2b和PR7。此外，用感染ϕRSM的细胞进行预处理可保护番茄植株免受强毒青枯雷尔氏菌菌株的感染。确定用于疾病预防的感染ϕRSM3细胞的有效剂量约为10 CFU/ml。由于感染ϕRSM3的细胞在适当条件下能够生长并持续产生有感染性的噬菌体颗粒，ϕRSM噬菌体可能成为防治作物青枯病的有效工具。
