Jen Fan-Yu, Tsay Tung-Tsuan, Chen Peichen
Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, 40223.
Plant Dis. 2012 Dec;96(12):1763-1766. doi: 10.1094/PDIS-03-12-0229-RE.
Aphelenchoides bicaudatus nematodes were identified in planting materials and water samples from 35 floral ornamental nurseries in Taiwan. A pure line of the nematode was established from the bulk samples collected, and the nematode was identified as A. bicaudatus on the basis of morphometric data and the 18S gene sequence (GU984233). Host range tests were conducted by inoculating A. bicaudatus onto Phalaenopsis spp. strawberry, rice, bird's-nest fern, cyclamen, dendrobium, African violet, and saxifrage (all reported hosts of A. besseyi). Symptoms did not develop during the 4 weeks following inoculation. However, after staining, both eggs and nematodes inside plant tissue of Phalaenopsis roots, strawberry leaf buds, and rice sheath indicated that A. bicaudatus had established in these hosts. These results revealed a potential new host-parasite relationship of A. bicaudatus, and suggest that this nematode may infect these economically important hosts.
在台湾35家花卉观赏苗圃的种植材料和水样中鉴定出了双尾滑刃线虫。从采集的大量样本中建立了该线虫的纯系，并根据形态测量数据和18S基因序列（GU984233）将该线虫鉴定为双尾滑刃线虫。通过将双尾滑刃线虫接种到蝴蝶兰属植物、草莓、水稻、鸟巢蕨、仙客来、石斛、非洲紫罗兰和虎耳草（均为贝西滑刃线虫的报道寄主）上进行寄主范围测试。接种后4周内未出现症状。然而，染色后，蝴蝶兰根部、草莓叶芽和水稻叶鞘的植物组织内的卵和线虫均表明双尾滑刃线虫已在这些寄主中定殖。这些结果揭示了双尾滑刃线虫潜在的新寄主-寄生关系，并表明这种线虫可能感染这些具有重要经济价值的寄主。
