Santos E C, Silva I L Da, Rosa R O, Oliveira C M G, Buonicontro D S
Viçosa Federal University, Department of Plant Pathology, Laboratory of Nematology, 36570-900, Viçosa, MG, Brazil.
Viçosa Federal University, Department of Plant Biology, Plant Anatomy Laboratory, 36570-900, MG, Brazil.
Helminthologia. 2021 Feb 10;58(1):74-84. doi: 10.2478/helm-2021-0003. eCollection 2021 Mar.
has recently been reported in association with seeds of forage grasses and rice in Brazil and senescent strawberry plants, in the United States. This nematode is likely a mycophagous species; however, so far, its pathogenicity potential to plants is unclear. This study aimed to verify the pathogenicity of to two species of ornamental plants. The experiments were conducted by inoculating onto Bird's-Nest Fern () and Oriental Lily () leaves, using two inoculation methods (with and without injury). After 40 days of inoculation (DAI) in Bird's-Nest Fern and 5, 10, 20 and 40 DAI in Oriental Lily, the pathogenicity and the host efficiency were evaluated by symptoms observation and by severity, final nematode population and reproductive factor (RF), respectively. Additionally, a histopathological study was performed by inoculating onto Bird's-Nest Fern for observing anatomical alterations. was able to cause local necrotic lesions on both Bird's-Nest Fern and Oriental Lily leaves. However, the presence of injury was essential to enable to penetrate and cause those symptoms in both plant species. Also, the total population of decreased drastically over time and RF was <1, which characterized these species as poor-host or resistant plants. penetrated into the foliar tissue and induced a total destruction of the mesophyll and collapse of the cells, with the formation of large intercellular spaces. It is concluded that is an opportunistic pathogen as injury was required to induce symptoms in Bird's-Nest Fern and Oriental Lily.
最近在巴西，该线虫与牧草种子和水稻有关，在美国则与衰老的草莓植株有关。这种线虫可能是一种食真菌物种；然而，到目前为止，其对植物的致病潜力尚不清楚。本研究旨在验证该线虫对两种观赏植物的致病性。实验通过两种接种方法（有损伤和无损伤）将该线虫接种到鸟巢蕨（）和东方百合（）叶片上进行。在鸟巢蕨接种40天后以及在东方百合接种5、10、20和40天后，分别通过症状观察以及严重程度、最终线虫种群和繁殖因子（RF）来评估致病性和寄主效率。此外，通过将该线虫接种到鸟巢蕨上进行组织病理学研究以观察解剖学变化。该线虫能够在鸟巢蕨和东方百合叶片上引起局部坏死病变。然而，损伤的存在对于该线虫穿透并在两种植物物种中引起这些症状至关重要。而且，该线虫的总种群数量随时间急剧下降，繁殖因子小于1，这表明这些物种是不良寄主或抗性植物。该线虫穿透到叶组织中，导致叶肉完全破坏和细胞塌陷，并形成大的细胞间隙。结论是，由于在鸟巢蕨和东方百合中诱导症状需要损伤，所以该线虫是一种机会性病原菌。
