Gat Tami, Liarzi Orna, Skovorodnikova Yulia, Ezra David
Department of Plant Pathology and Weed Research, ARO The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel.
Plant Dis. 2012 Oct;96(10):1513-1518. doi: 10.1094/PDIS-12-11-1041-RE.
Black spot disease of pomegranate is a relatively new disease in Israel that is caused by Alternaria alternata. The symptoms include black spots on leaves and fruit. Only the outer part of the fruit is damaged; the edible tissue remains unaffected. In this study, we obtained 50 isolates of A. alternata from infected pomegranate plants that were classified based on pathogenicity tests using detached leaves. Using an arbitrarily primed polymerase chain reaction, we identified one primer (primer CAG) that reacted only with DNA of isolates that induced the most severe disease symptoms. Based on the sequence of the amplified fragment, we generated a specific primer (primer C) that recognizes these highly virulent isolates. Therefore, we suggest that primer C can be utilized as a molecular marker for the detection of A. alternata isolates that cause black spot disease of pomegranate.
石榴黑斑病是以色列一种相对较新的疾病，由链格孢引起。症状包括叶片和果实上出现黑斑。仅果实外部受损，可食用组织未受影响。在本研究中，我们从受感染的石榴植株上获得了50株链格孢分离株，通过使用离体叶片进行致病性测试对其进行分类。利用任意引物聚合酶链反应，我们鉴定出一种引物（引物CAG），它仅与诱导最严重病害症状的分离株的DNA发生反应。基于扩增片段的序列，我们生成了一种特异性引物（引物C），可识别这些高毒力分离株。因此，我们建议引物C可作为一种分子标记，用于检测引起石榴黑斑病的链格孢分离株。
