Lakshmanan P, Lee C L, Goh C J
Plant Tissue Culture Laboratory, School of Biological Sciences, National University of Singapore, Lower Kent Ridge Road, 119260, Singapore.
Plant Cell Rep. 1997 May;16(8):572-577. doi: 10.1007/BF01142326.
Various factors that affect culture establishment, shoot growth, proliferation and rooting ofIxora coccinea L., a woody shrub, were studied. Stem cuttings (decapitated shoot, three nodes) were the most suitable explants for multiple-shoot proliferation, and when cultured on a woody plant medium (WPM) containing 2.5 μM BA produced axillary shoots which branched repeatedly, yielding an average of 27 shoots per explant after 6 weeks in culture. Kinetin, 2-iP, zeatin and thidiazuron all induced multiple-shoot formation, but were less effective than BA. While the presence of IAA in the multiplication medium was detrimental to shoot proliferation, shoot growth was not affected by IAA. The production of large amounts of basal callus and vitrification of shoots were the major problems to be avoided in proliferating shoot cultures. Addition of TIBA to the multiplication medium markedly reduced basal callusing, while sealing the culture vessels with a fluorocarbon polymer (tetrafluoroethyleneperfluoroalkyl vinyl ether) film (Neoflon PFA film) almost completely eliminated vitrification. A reduction in the number of vitrified shoots was also achieved with AVG treatment. Following this protocol of using BA-supplemented WPM and Neoflon film, it would be possible to produce more than 100,000 plants from a single stem cutting in 1 year.
对影响木本灌木龙船花的培养建立、芽生长、增殖和生根的各种因素进行了研究。茎段插穗（去顶芽，带三个节）是最适合进行多芽增殖的外植体，当在含有2.5 μM 苄氨基嘌呤（BA）的木本植物培养基（WPM）上培养时，能产生反复分枝的腋芽，培养6周后每个外植体平均产生27个芽。激动素、异戊烯腺嘌呤、玉米素和噻苯隆都能诱导多芽形成，但效果不如BA。虽然增殖培养基中吲哚乙酸（IAA）的存在对芽增殖不利，但芽的生长不受IAA影响。在增殖芽培养中，大量基部愈伤组织的产生和芽的玻璃化是需要避免的主要问题。在增殖培养基中添加三碘苯甲酸（TIBA）可显著减少基部愈伤组织的形成，而用碳氟聚合物（四氟乙烯全氟烷基乙烯基醚）薄膜（Neoflon PFA薄膜）密封培养容器几乎完全消除了玻璃化现象。用氨基乙氧基乙烯基甘氨酸（AVG）处理也能减少玻璃化芽的数量。按照使用添加BA的WPM和Neoflon薄膜的方案，一年内从单个茎段插穗有可能生产出超过100,000株植株。
