Teng W-L, Teng M-C
Hong Kong Institute of Biotechnology, 2 Biotechnology Avenue, 12 Miles, Tai Po Road, Shatin, NT, Hong Kong Fax no.: +852-26036820 E-mail:
Department of Plant Industry, National Pingtung Polytechnic Institute, 1 Hseuh Fu Road, Nei Pu Hsiang, Pingtung, Taiwan, , , , , , TW.
Plant Cell Rep. 1997 Oct;16(12):820-824. doi: 10.1007/s002990050327.
A simple suspension culture system of Platycerium bifurcatum was developed where sporophytes could be regenerated directly from leaf cells or indirectly through an aposporous gametophyte stage under the same culture conditions. Single cells and aggregates of up to 100 cells developed aposporous gametophytes which later gave rise to sporophytes. Such gametophytes started apogamy when they were mostly less than 0.7 mm in length, bearing only rhizoids. In most cases, only one sporophyte was regenerated from one gametophyte. Aggregates of 500-1000 or more cells, on the other hand, regenerated sporophytes directly. Intercellular interaction was considered to be the physiological cause, and the separation of leaf cells to a certain degree drove the cells to embark on different regeneration paths. It is suggested that the possible existence of a threshold size of cell aggregates separates the two regeneration patterns.
开发了一种简单的叉叶鹿角蕨悬浮培养系统，在相同培养条件下，孢子体可以直接从叶细胞再生，也可以通过无孢子配子体阶段间接再生。单个细胞和多达100个细胞的聚集体发育出无孢子配子体，随后产生孢子体。当这些配子体大多长度小于0.7毫米且仅具假根时，开始进行无配子生殖。在大多数情况下，一个配子体仅再生出一个孢子体。另一方面，500 - 1000个或更多细胞的聚集体直接再生出孢子体。细胞间相互作用被认为是生理原因，叶细胞在一定程度上的分离促使细胞走上不同的再生途径。有人提出，细胞聚集体可能存在的阈值大小将两种再生模式区分开来。
