Polish Academy of Sciences, Botanical Garden-Center for Biology Diversity Conservation in Powsin, 2 Prawdziwka Str., 02-973 Warsaw, Poland.
Department of Cytophysiology, Łódź University, 90-236 Lódź, Poland.
Cells. 2022 Apr 20;11(9):1396. doi: 10.3390/cells11091396.
The aim of our research was to describe the structure and growth potential of a cell suspension of the tree fern . Experiments were performed on an established cell suspension with ½ MS medium supplemented with 9.05 µM 2,4-D + 0.88 µM BAP. In the experiments, attention was paid to the microscopic description of cell suspension, evaluation of cell growth dependent on the initial mass of cells and organic carbon source in the medium, the length of the passage, the content of one selected flavonoid in the post-culture medium, nuclear DNA content, ethylene production, and the antimicrobial value of the extract. For a better understanding of the cell changes that occurred during the culture of the suspension, the following structures of the cell were observed: nucleus, lipid bodies, tannin deposits, starch grains, cell walls, primary lamina, and the filaments of metabolites released into the medium. The nuclear DNA content (acriflavine-Feulgen staining) of cell aggregates distinctly indicated a lack of changes in the sporophytic origin of the cultured cell suspension. The physiological activity of the suspension was found to be high because of kinetics, intensive production of ethylene, and quercetin production. The microbiological studies suggested that the cell suspension possessed a bactericidal character against microaerobic Gram-positive bacteria. A sample of the cell suspension showed bacteriostatic activity against aerobic bacteria.
我们研究的目的是描述树蕨细胞悬浮液的结构和生长潜力。实验是在一个建立的细胞悬浮液中进行的，该悬浮液使用 ½ MS 培养基补充 9.05 µM 2,4-D + 0.88 µM BAP。在实验中，我们关注细胞悬浮液的微观描述、细胞生长与细胞初始质量和培养基中有机碳源的关系、传代长度、后培养介质中一种选定类黄酮的含量、核 DNA 含量、乙烯产生以及提取物的抗菌值。为了更好地理解悬浮培养过程中细胞发生的变化，观察了以下细胞结构：细胞核、脂体、单宁沉积物、淀粉颗粒、细胞壁、初生叶和代谢产物释放到介质中的丝状结构。细胞核 DNA 含量（吖啶橙-Feulgen 染色）明显表明培养细胞悬浮液的孢子体起源没有变化。由于动力学、乙烯的大量产生和槲皮素的产生，悬浮液的生理活性被发现很高。微生物学研究表明，细胞悬浮液对微需氧革兰氏阳性菌具有杀菌特性。细胞悬浮液的样品对需氧菌表现出抑菌活性。
