Kim S J, Lee S J, Kim B-D, Paek K-H
Department of Horticulture, Seoul National University, Suwon 441-744, Korea 2, , , , , , KR.
Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea Fax no.: +82-2-928-1274 E-mail:
Plant Cell Rep. 1997 Oct;16(12):825-830. doi: 10.1007/s002990050328.
We previously established a system of in vitro regeneration and Agrobacterium-mediated transformation for hot pepper plants. The level of protection against cucumber mosaic virus in the progeny of the transgenic hot pepper plants that express cucumber mosaic virus (CMV) satellite RNA was investigated. The transgenic hot pepper plants were self-fertilized, and their progeny were tested for stable inheritance and expression of the cDNA of CMV satellite RNA. Polymerase chain reaction and RNA gel blot analyses showed that the introduced gene was stably transmitted and expressed in the progeny. Symptom attenuation in the offspring was confirmed upon inoculation with CMV-Y or CMV-Korea (CMV-Kor) strains.
我们之前建立了一个用于辣椒植株的体外再生和农杆菌介导转化系统。对表达黄瓜花叶病毒（CMV）卫星RNA的转基因辣椒植株后代中针对黄瓜花叶病毒的保护水平进行了研究。转基因辣椒植株进行自花授粉，并对其后代进行CMV卫星RNA cDNA的稳定遗传和表达检测。聚合酶链反应和RNA凝胶印迹分析表明，导入的基因在后代中稳定传递并表达。在用CMV-Y或CMV-韩国（CMV-Kor）株系接种后，证实了后代中的症状减轻。
